单核细胞增生李斯特菌对季铵盐类消毒剂的耐受机制及其防控研究进展  

Research progress on the resistance of foodborne Listeria monocytogenes to quaternary ammonium disinfectants and its prevention and control strategies

作　　者：王振华[1] 李港 张敏[1] 王晔茹 WANG Zhenhua;LI Gang;ZHANG Min;WANG Yeru(Beijing Advanced Innovation Center for Food Nutrition and Human Health,School of Food and Health,Beijing Technology and Business University,Beijing 100048,China;China National Center for Food Safety Risk Assessment,Beijing 100022,China)

机构地区：[1]北京工商大学食品与健康学院、北京食品营养与人类健康高精尖创新中心,北京100048 [2]国家食品安全风险评估中心,北京100022

出　　处：《中国食品卫生杂志》2023年第2期294-302,共9页Chinese Journal of Food Hygiene

基　　金：“十三五”国家重点研发计划(2019YFC1605900)。

摘　　要：单核细胞增生李斯特菌(以下简称“单增李斯特菌”)是一种常见的食源性病原菌,可通过群体感应在不锈钢食品加工设备表面形成单一或多物种生物膜,成为食品潜在的污染源。季铵盐类消毒剂常用于食品企业加工设备表面的清洗消毒,而单增李斯特菌生物膜在主动外排泵系统、应激反应、基因调控等多种机制的介导下可对其产生耐受性。本文综述了单增李斯特菌生物膜的形成及耐受机制,汇总了近年来物理杀菌以及新型组合杀菌技术在食源性单增李斯特菌防控中的应用,为新型高效杀菌技术的开发提供思路,也为企业建立单增李斯特菌及其他微生物防控措施提供重要参考。Listeria monocytogenes(L.monocytogens)is a common foodborne pathogen that can become a potential source of food contamination by forming single or multi-species biofilms through population induction on the surface of stainless steel of food processing equipment.Quaternary disinfectants are commonly used for cleaning and disinfection of processing equipment surfaces in food plant,while L.monocytogenes biofilms are tolerant to it through various mechanisms,such as active efflux pump system,stress response and gene regulation.In this paper,the formation and tolerance mechanisms of L.monocytogenes biofilms are reviewed,and the applications of physical disinfection and new combined disinfection technologies for foodborne L.monocytogenes prevention and control in recent years are summarized to provide ideas for the development of new and efficient disinfection technology,as well as to supply important references for enterprises to establish the control measures for L.monocytogenes and other microbial.

关 键 词：季铵盐 单核细胞增生李斯特菌 生物膜 耐受机制 杀菌 

分 类 号：R155[医药卫生—营养与食品卫生学]