聚乙烯醇/马齿苋提取物保鲜膜对猪肉的抑菌保鲜效果研究  

Study on bacteriostasis and preservation effectiveness of poly(vinyl alcohol)/Portulaca oleracea extract fresh⁃keeping films for pork

作　　者：白静 李明 崔丽伟[2] BAI Jing;LI Ming;CUI Liwei(Henan Vocational College of Applied Technology,Zhengzhou 450000,China;Henan Animal Husbandry and Economics College,Zhengzhou 450001,China)

机构地区：[1]河南应用技术职业学院,郑州450000 [2]河南牧业经济学院,郑州450001

出　　处：《中国塑料》2023年第4期46-52,共7页China Plastics

基　　金：河南省高等学校重点科研项目(23A550021);河南应用技术职业学院2020年度青年骨干教师项目(2020-GGJS-H001)。

摘　　要：针对猪肉极易受到微生物污染、货架期较短的缺点,以马齿苋为原料采用酶解-超声波辅助乙醇法对其黄酮类化合物进行提取,研究马齿苋提取液抗氧化抑菌活性的影响。马齿苋提取液与聚乙烯醇(PVA)复合膜应用于猪肉的保鲜试验中,验证其抑菌保鲜效果。结果表明,马齿苋黄酮提取液对羟基(OH)、羟基、a-二苯基联吡啶肼(DPPH)自由基均具有清除能力,并且对DPPH自由基的清除率在黄酮浓度为0.02 mg/mL就达到了90%以上。对金黄色葡萄球菌有较大的抑制作用。PVA与马齿苋提取液比例为30∶20的保鲜膜,能显著抑制冷却肉的菌落总数、酸度值(p H值)、硫化氢(H2S)、丙二醛、挥发性盐基氮(TVB-N)含量的增长速率,延长其货架期。Aiming at the easy contamination of pork by microorganisms along with a short shelf life,flavonoid compounds were extracted from Purslane through enzymatic hydrolysis,followed by ultrasonic⁃assisted alcohol extraction,and the an⁃tioxidant and bacteriostatic activity of the resultant Portulaca oleracea extract was investigated.The Portulaca oleracea ex⁃tract and PVA composite film were applied for the preservation of pork to verify its bacteriostasis and preservation effects.The results indicated that the flavone extract of Portulaca oleracea L.had a scavenging activity on the OH and DPPH free radicals,and the scavenging rate of DPPH free radical was over 90%at a flavone concentration of 0.02 mg/mL,exhibit⁃ing a high bacteriostasis effect on Staphylococcus aureus.When the ratio of Portulaca oleracea extract to PVA was 20∶30,the growth rates of total colony count,pH,H2S,MDA and TVB⁃N contents of chilled meat could be significantly inhibit⁃ed,prolonging its shelf life.

关 键 词：黄酮类化合物 抗氧化能力 抑菌 复合膜 保鲜 

分 类 号：TQ325.9[化学工程—合成树脂塑料工业]