2020—2022年上海市浦东新区市售食品中沙门菌耐药特征研究  

Serotypes and drug resistance of salmonella in commercial food in Pudong New Area from 2020 to 2022

作　　者：梁艳茹 陈诺 杨博雯 王筱 丁锦 桂燕华 邱惠芳[1] 郝莉鹏 潘丽峰[1,3] LIANG Yanru;CHEN Nuo;YANG Bowen;WANG Xiao;DING Jin;GUI Yanhua;QIU Huifang;HAO Lipeng;PAN Lifeng(Shanghai Pudong New Area Center for Disease Control and Prevention,Shanghai 200136,China;Shanghai University of Medicine and Health Science,Shanghai 200025,China;Fudan University Pudong Institute of Preventive Medicine,Shanghai 200136,China)

机构地区：[1]上海市浦东新区疾病预防控制中心,上海200136 [2]上海健康医学院,上海200025 [3]复旦大学浦东预防医学研究院,上海200136

出　　处：《上海预防医学》2023年第4期374-379,共6页Shanghai Journal of Preventive Medicine

基　　金：上海市浦东新区卫生健康委公共卫生高原学科—病原微生物检验基金项目(PWYggy2021-01);上海市浦东新区疾病预防控制中心“后备学科带头人”人才培养基金项目(PDCDC-HBXD2020-04)。

摘　　要：【目的】了解市售食品中沙门菌污染情况、血清型分布以及耐药情况。【方法】对2020—2022年浦东新区抽检的市售食品进行沙门菌检测,并对所获菌株开展血清学分型和15种抗生素的微量肉汤法药敏试验。【结果】2497件食品共检出118株沙门菌,总检出率为4.7%,优势检出类别依次为禽肉类、畜肉类和水产类。118株沙门菌可分为24种血清型,肠炎沙门菌(26.4%)、鼠伤寒沙门菌(16.2%)和德尔卑沙门菌(14.4%)是主要优势型别。沙门菌对氨苄西林耐药率最高,达63.6%,其次是四环素、氯霉素、复方新诺明和萘啶酸。3种优势血清型中鼠伤寒沙门菌多重耐药率最高为89.5%,其次是德尔卑沙门菌(70.6%)和肠炎沙门菌(61.3%)。【结论】市售食品中,畜、禽肉与水产品沙门菌污染严重,血清型别多样化,畜肉以鼠伤寒、德尔卑沙门菌为主,禽肉以肠炎沙门菌为主;耐药谱广泛,多重耐药率高,不同于畜肉、水产类分离株,禽肉源菌株对氨苄西林、萘啶酸和多黏菌素的耐受较高,具有一定的食品安全隐患。[Objective]To understand the serotype distribution and drug resistance of salmonella contaminated in commercially available food.[Methods]Salmonella detection,including the serotypes,was conducted in food products sold in Pudong New Area from 2020 to 2022.The antimicrobial susceptibility test of 15 antibiotics was conducted by the broth microassay.[Results]A total of 118 salmonella strains were detected in 2497 pieces of food,with a total detection rate of 4.7%.The dominant detection categories were poultry meat,livestock meat and aquatic products.The 118 salmonella strains could be divided into 24 serotypes,Salmonella enteritidis(26.4%),Salmonella Typhimurium(16.2%)and Salmonella delpy(14.4%)were the main dominant types.Salmonella had the highest resistance rate to ampicillin(63.6%),followed by tetracycline,chloramphenicol,cotrimoxazole and nalidixic acid.Among the three dominant serotypes,the multidrug resistance rate of Salmonella typhimurium was the highest(89.5%),followed by Salmonella delpy(70.6%)and Salmonella enteritidis(61.3%).[Conclusion]Livestock,poultry meat,and aquatic products are seriously contaminated by salmonella with diverse serotypes.The livestock meat is mainly contaminated by Salmonella typhimurium and Salmonella delpy,and the poultry meat is mainly contaminated by Salmonella enteritidis.The drug resistance spectrum is wide and the multi-drug resistance rate is high.Different from the livestock and aquatic isolates,poultry meat-derived strains have high tolerance to ampicillin,nalidixic acid and polymyxin,and carry certain potential food safety risks.

关 键 词：沙门菌 血清型 耐药 食源性疾病 市售食品 

分 类 号：R15[医药卫生—营养与食品卫生学]