甜茶甙对口腔变异链球菌生长及致龋性影响的实验研究  

Experimental Study on the Effect of Rubusoside on the Growth and Cariogenicity Induced by Streptococcus Mutans

作　　者：杨淑银 岳二丽 郭留云 陆珂 杨梦宇 YANG Shuyin;YUE Erli;GUO Liuyun;LU Ke;YANG Mengyu

机构地区：[1]郑州大学第一附属医院牙周科,河南郑州450052

出　　处：《新中医》2023年第7期9-14,共6页New Chinese Medicine

基　　金：河南省高等学校重点科研计划项目(20B320053)。

摘　　要：目的:探讨甜茶甙对口腔变异链球菌(S.mutans)生长及致龋性的影响,为龋齿的防治提供新思路。方法:S.mutans被处理后于恒温厌氧环境下培养,检测菌液OD值筛选最小抑菌浓度(MIC),并确定0.625、1.25、2.5、5 mg/mL为工作浓度。检测工作浓度下甜茶苷和木糖醇培养S.mutans菌液pH、疏水率、黏附力、水不溶性多聚糖(WIG)合成力及葡萄糖基转移酶(GTF)活性指标——总蛋白含量、还原糖量、GTF酶活力及比活力,并比较分析。结果:甜茶甙≥0.625 mg/mL时可明显抑制S.mutans菌的生长,其MIC为10 mg/mL;与木糖醇组及对照组相比,加入甜茶甙后S.mutans的产酸、黏附力、疏水率及WIG、还原糖量、GTF酶活力、比活力均降低,总蛋白含量增高(P<0.05),且均具有浓度依懒性。与对照组相比,木糖醇组仅在浓度≥2.5 mg/mL时对S.mutans的疏水率、黏附力及WIG、还原糖量、酶活力、比活力有明显抑制作用(P<0.05),但对产酸能力和总蛋白含量无明显影响(P>0.05)。结论:甜茶甙可有效抑制S.mutans的生长、产酸、黏附、WIG合成及GTF活性等致龋特性,其与浓度相关,同浓度下效果优于木糖醇。Objective:To investigate the effects of rubusoside on the growth and cariogenicity of streptococcus mutans(S.mutans),and to provide a new idea for the prevention and treatment of dental caries.Methods:S.mutans was treated and cultured in a constant temperature and anaerobic environment,and the minimum inhibitory concentration(MIC)was determined by the OD value of bacterial solution,and 0.625,1.25,2.5 and 5 mg/mL were determined as working concentrations.pH,hydrophobicity,adhesion,biosynthesis of water-insoluble polysaccharide(WIG)and glucosyltransferase(GTF)activity indexes including total protein content,reducing sugar content,GTF activity and specific activity of S.mutans cultured with rubusoside or xylitol at working concentration were detected and compared.Results:The growth of S.mutans was significantly inhibited by rubusoside at concentration≥0.625 mg/mL,and its MIC was 10 mg/mL.Compared with the control group and xylitol group,acid production,hydrophobicity,adhesion,WIG,reducing sugar content,GTF activity and specific activity of S.mutans were decreased after addition of rubusoside,and the total protein content was increased(P<0.05).Compared with the control group,xylitol group had obvious inhibitory effects on hydrophobic rate,adhesion and WIG,reducing sugar content,enzyme activity and specific activity of S.mutans only when the concentration was≥2.5 mg/mL(P<0.05),but had no significant effects on acid-producing capacity and total protein content(P>0.05).Conclusion:Rubusoside can effectively inhibit the growth,acid production,adhesion,WIG synthesis and GTF activity of S.mutans,which is better than xylitol at the same concentration.

关 键 词：变异链球菌 甜茶甙 木糖醇 致龋性 葡萄糖基转移酶 

分 类 号：R781.1[医药卫生—口腔医学]