酶法合成共轭亚油酸的研究进展  

Research Progress on Enzymatic Synthesis of Conjugated Linoleic Acid

作　　者：赵微 师文卓 曹然 崔美林 张秀红 赵国芬[2] ZHAO Wei;SHI Wen-zhuo;CAO Ran;CUI Mei-lin;ZHANG Xiu-hong;ZHAO Guo-fen(College of Food Science,Shanxi Normal University,Taiyuan 030031,Shanxi,China;College of Life Sciences,Inner Mongolia Agricultural University,Hohhot 010011,Inner Mongolia,China)

机构地区：[1]山西师范大学食品科学学院,山西太原030031 [2]内蒙古农业大学生命科学学院,内蒙古呼和浩特010011

出　　处：《食品研究与开发》2023年第9期211-217,共7页Food Research and Development

基　　金：山西省基础研究计划(202103021223255)。

摘　　要：共轭亚油酸(conjugated linoleic acid,CLA)是一类具有抗癌、抗动脉粥样硬化和降脂等多种生理活性的功能性脂肪酸的统称,在食、药和畜牧业领域极具应用潜力。CLA的天然来源有限,主要依赖于化学法和生物法合成。利用生物法合成的CLA,异构体单一且食用安全,是合成CLA方式的最佳选择。其中,利用酶法合成CLA因具有产物高纯度和高含量、合成高效率等优势,成为当今研究热点。该文对酶法合成CLA途径以及合成途径中关键酶的研究现状进行综述,为提高生物合成CLA效率提供参考。Conjugated linoleic acid(CLA)is a general term for a class of functional fatty acids with anti-cancer,anti-atherosclerosis,lipid-lowering,and various other physiological activities.It has great application potential in the fields of food,medicine and animal husbandry.Due to limited natural sources,CLA is mainly synthesized by chemical and biological methods.CLA by biological synthesis has single isomers with high safety,which is the optimal choice for CLA synthesis.Among the biological methods,enzymatic synthesis of CLA is currently a research hotspot for the high purity and high content of products and high synthesis efficiency.In this study,the enzymatic synthesis pathway of CLA and the key enzymes in the pathway were reviewed,to provide guidance for improving the efficiency of CLA biosynthesis.

关 键 词：共轭亚油酸 亚油酸异构酶 亚油酸水合酶 羟基脂肪酸脱氢酶 乙酰乙酸脱羧酶 α-烯醇化酶 

分 类 号：TS201.2[轻工技术与工程—食品科学]