羊血亚铁血红素的酶法制备及抗氧化活性研究  

Enzymatic Preparation and Antioxidant Activity of Ferroheme from Sheep Blood

作　　者：韩晴 王维婷[2] 王守经[2] 柳尧波[2] 杜鹏飞 温蕾 江利华 马艳丽 HAN Qing;WANG Weiting;WANG Shoujing;LIU Yaobo;DU Pengfei;WEN Lei;JIANG Lihua;MA Yanli(Laboratory of Natural Products and Functional Food Development,Life Sciences and Food Engineering,Hebei University of Engineering,Handan 056038,China;Institute of Food&Nutrition Science and Technology,Shandong Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing Technology of Shandong Province,Key Laboratory of Novel Food Resources Processing,Ministry of Agriculture,Ji’nan 250100,China;College of Life Sciences,Yantai University,Yantai 264005,China)

机构地区：[1]河北工程大学生命科学与食品工程学院,邯郸市天然产物与功能食品开发重点实验室,河北邯郸056038 [2]山东省农业科学院农产品加工与营养研究所,山东省农产品精深加工技术重点实验室,农村农业部新食品资源加工重点实验室,山东济南250100 [3]烟台大学生命科学学院,山东烟台264005

出　　处：《食品科技》2023年第3期147-154,共8页Food Science and Technology

基　　金：山东省农业科学院农业创新工程项目(CXGC2022E09);山东省现代农业产业技术体系-羊创新团队建设项目(SDAIT-10-10);国家现代农业(肉羊)产业技术体系建设专项(CARS-38);山东省乡村振兴科技创新提振行动计划项目(2021TZXD012);山东省自然科学青年基金项目(ZR2020QC250)。

摘　　要：以新鲜羊血为原料,通过酶水解法制备亚铁血红素,并测定其体外抗氧化活性。以水解度为评价指标,对比分析中性蛋白酶、木瓜蛋白酶、Alcalase2.4L碱性蛋白酶酶解效果,确定选用Alcalase2.4L碱性蛋白酶为水解酶。通过单因素实验及正交实验得到最优酶解工艺为:温度55℃、pH8.5、加酶量8000 U/g,酶解时间8 h。添加0.05%抗坏血酸抗氧化,最终血红素得率为94.65%,其中亚铁血红素纯度为83.87%。以Vc为阳性对照,通过测定亚铁血红素的DPPH自由基清除率、羟自由基清除率、ABTS自由基清除率研究其抗氧化活性,结果表明亚铁血红素有较好的抗氧化活性。In this study,heme was prepared by enzymatic hydrolysis from fresh sheep blood and its antioxidant activity was measured in vitro.With the degree of hydrolysis as the evaluation index,the enzymatic hydrolysis effects of neutral protease,papain and Alcalase 2.4L alkaline protease were compared and analyzed,and the Alcalase 2.4L alkaline protease was selected as the hydrolase.Through single factor experiment and orthogonal experiment,the optimal enzymatic hydrolysis process was as follows:temperature 55℃,pH 8.5,enzyme amount 8000 U/g,enzymatic hydrolysis time 8 h.The final yield of heme was 94.65%and the purity of heme was 83.87%when 0.05%ascorbic acid was added.Using Vc as positive control,the antioxidant activity of heme was studied by measuring its DPPH radical scavenging rate,hydroxyl radical scavenging rate and ABTS radical scavenging rate.The results showed that heme had better antioxidant activity.

关 键 词：羊血 亚铁血红素 酶法 水解度 抗氧化 

分 类 号：TS251.93[轻工技术与工程—农产品加工及贮藏工程]