一种复合菌对疲劳小鼠肠黏膜屏障的影响及其作用机制  

Effects of A Compound Bacterium on Intestinal Mucosal Barrier in Fatigue Mice and Its Mechanism

作　　者：刘昕琦 陈芊汝 朴美子[1,2] 徐庆 高倩[1,2] 刘英华 LIU Xin-qi;CHEN Qian-ru;PARK Mei-zi;XU Qing;GAO Qian;LIU Ying-hua(College of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China;Qingdao Special Food Research Institute,Qingdao 266109,China;Department of Nutrition,First Medical Center,PLA General Hospital,Beijing 100853,China)

机构地区：[1]青岛农业大学食品科学与工程学院,山东青岛266109 [2]青岛特种食品研究院,山东青岛266109 [3]解放军总医院第一医学中心营养科,北京100853

出　　处：《中国食物与营养》2023年第4期62-67,共6页Food and Nutrition in China

基　　金：“十三五”国家重点研发计划“食品安全关键技术研发”专项(项目编号:2018YFC1602105);解放军总医院军事转化医学项目(项目编号:ZH19012);青岛特种食品研究院课题(项目编号:66120001)。

摘　　要：目的:通过负重游泳实验,观察一种复合菌对小鼠的抗疲劳作用及其在疲劳状态下对肠道粘膜屏障的影响,并初步探讨其机制。方法:50只昆明小鼠(4~6周龄)随机分为5组:空白组、疲劳模型组、嗜酸乳杆菌干预组、干酪乳杆菌干预组、复合菌干预组,每日进行灌胃、负重游泳。6 w后取小鼠眼球血及小肠组织,测定肠黏膜损伤程度相关指标、细胞因子水平和蛋白水平的表达。研究期间每周测量1次小鼠体重。结果:研究结束时,各组小鼠体重均升高,各组间无显著性差异(P>0.05);血清中肠黏膜物理屏障相关指标DAO、D-LA水平以复合菌组最低,其次是干酪乳杆菌组,与空白组和疲劳模型组比较均有显著性差异(P<0.05或<0.01),而Endotoxin水平各组间无显著差异(P>0.05)。血和小肠中炎性因子TNF-α、IFN-γ、IL-4、IL-10、MPO水平3个干预组与空白组和疲劳模型组相比均有显著性差异(P<0.05或<0.01),而IL-6、IL-1β、sIgA水平与疲劳模型组相比无显著性差异(P>0.05)。小肠中蛋白水平MUC2、ZO-1、Occludin、Claudin-2的表达3个干预组和疲劳模型组相比较均有显著性差异(P<0.05或<0.01)。结论:复合菌能显著延长小鼠力竭游泳时间;嗜酸乳杆菌与干酪乳杆菌均在不同程度上抑制了血清及肠道细胞因子的释放;促进了相应蛋白水平的表达;初步认为复合菌通过增加对小鼠肠道屏障的保护作用发挥抗疲劳作用。Objective To observe the anti-fatigue effect of a compound bacteria on mice and its effect on intestinal mucosal barrier under fatigue state through weight-bearing swimming experiment,and to preliminarily explore its mechanism.Method Totally 50 Kunming mice(4—6 weeks of age)were randomly divided into 5 groups including blank group,fatigue model group,Lactobacillus acidophilus intervention group,Lactobacillus casei intervention group and compound bacteria intervention group.After 6 w,the blood of the eye and small intestine of the mice were collected,and the expressions of cytokines and proteins in intestinal mucosal injury degree were determined.The mice were weighed weekly during the study.Result At the end of the study,the body weight of mice in all groups increased,but there was no significant difference among the groups(P>0.05).The DAO and D-La levels of serum physical barrier related indexes of midgut mucosa were the lowest in the compound bacteria group,followed by Lactobacillus casei group,compared with blank group and fatigue model group(P<0.05 or<0.01),but there was no significant difference in Endotoxin levels among groups(P>0.05).The levels of inflammatory factors TNF-α,IFN-γ,IL-4,IL-10 and MPO in blood and small intestine of the intervention group were significantly different from those of the blank group and the fatigue model group(P<0.05 or<0.01),while there were no significant differences in IL-6,IL-1βand sIgA levels between fatigue model group and fatigue model group(P>0.05).The expression of MUC2,ZO-1,Occludin and Claudin-2 protein levels in small intestine in the three intervention groups were significantly different from that in the fatigue model(P<0.05 or<0.01).Conclusion Compound bacteria can significantly prolong the exhaustive swimming time of mice.Both Lactobacillus acidophilus and Lactobacillus casei inhibited the release of serum and intestinal cytokines to varying degrees.It promoted the expression of corresponding protein level.It is preliminarily concluded that compound bacteria

关 键 词：疲劳 肠黏膜屏障 嗜酸乳杆菌 干酪乳杆菌 炎性因子 

分 类 号：TS201.4[轻工技术与工程—食品科学]