脱支酶解-压热法制备淮山薯RS3抗性淀粉的工艺优化及体外消化模拟评价  

Process optimization and in vitro digestion simulation evaluation of Dioscorea opposita Thunb.RS3 resistant starch prepared by debranching enzymolysis⁃pressure heat method

作　　者：孙国勇[1] 左映平[1] 丁仪 陈嘉丽 王夏琪 SUN Guo-yong;ZUO Ying-ping;DING Yi;CHEN Jia-li;WANG Xia-qi(Maoming Polytechnic,Maoming 525000,Guangdong,China)

机构地区：[1]茂名职业技术学院,广东茂名525000

出　　处：《粮食与油脂》2023年第5期58-61,共4页Cereals & Oils

基　　金：2021年广东省科技创新战略专项资金(大学生科技创新培育)项目(pdjh2021b0983);2021年广东省科技创新战略和乡村振兴战略专项资金(“大专项+任务清单”)项目(2021S0058);广东省教育厅2019年度普通高校认定类科研项目立项项目(2019GKTSCX125)。

摘　　要：以淮山薯为原料,通过脱支酶解-压热法制备RS_(3)抗性淀粉。以抗性淀粉得率为指标,在单因素试验的基础上通过正交试验优化制备工艺,并通过体外消化模拟试验评价RS_(3)抗性淀粉的消化性能。结果表明:最佳制备工艺为淀粉乳质量分数25%、普鲁兰酶添加量200 U/g(以干基淀粉质量计)、酶解时间12 h、老化时间18 h,在此条件下RS_(3)抗性淀粉得率为16.95%±0.22%;淀粉还原糖释放量为11.35%±0.20%,RS_(3)抗性淀粉还原糖释放量为8.42%±0.14%(P<0.05),表明RS_(3)抗性淀粉比淀粉抗消化能力更强。Using Dioscorea opposita Thunb.as raw material,RS_(3) resistant starch was prepared by de⁃branching enzymolysis⁃pressure heat method.Based on the yield of RS_(3) resistant starch as an indicator,the preparation process was optimized through orthogonal experiments on the basis of single factor experi⁃ments,and the digestion performance of RS_(3) resistant starch was evaluated through in vitro digestion sim⁃ulation experiments.The results showed that the best preparation technology was as follows:starch milk mass fraction 25%,pullulanase addition 200 U/g(based on dry starch mass),enzymolysis time 12 h,aging time 18 h.Under these conditions,the yield of RS_(3) resistant starch was 16.95%±0.22%.The release of starch reducing sugar was 11.35%±0.20%,and the release of RS_(3) resistant starch reducing sugar was 8.42%±0.14%(P<0.05),indicating that RS_(3) resistant starch has stronger digestive resistance than starch.

关 键 词：淮山薯 抗性淀粉 脱支酶解-压热法 

分 类 号：TS235.2[轻工技术与工程—粮食、油脂及植物蛋白工程]