寒地越冬模式下的羊肚菌生产菌株筛选  

Screening Study of the Morchella esculenta Production Strains in the Overwintering Model in Cold Regions

作　　者：马庆芳[1] 张丕奇[1] 马银鹏[1] 戴肖东[1] 田洁萍[1] 刘佳宁[1] MA Qingfang;ZHANG Piqi;MA Yinpeng;DAI Xiaodong;TIAN Jieping;LIU Jianing(Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin,Heilongjiang 150010)

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010

出　　处：《北方园艺》2023年第8期115-120,共6页Northern Horticulture

基　　金：黑龙江省科学院中试熟化资助项目(ZS2021SW01);黑龙江省科学院双提雁阵资助项目(YZ202002);黑龙江省科学院青年创新基金杰青资助项目(CXJQ2022SW01);省部共建食用菌产业技术创新中心平台建设资助项目(SJKYYWFC2021SW02);食用菌种质资源体系建设及特色高值品种栽培技术创新研究资助项目(YZ2022SW01)。

摘　　要：以引进的22株羊肚菌生产菌株为试材,对比不同菌株cPDA平板培养基和栽培种培养基菌丝生长情况,寒地条件下暖棚内采用“秋播春收”模式,研究了cPDA培养基和栽培种培养基生长情况,并进行栽培比较试验,以期选育出适于寒冷地区栽培的优良生产菌株。结果表明:22个生产菌株,依据cPDA平板培养基生长情况可分为3个组群;依据栽培种生长情况可分为2个组群;从栽培试验结果看,9号菌株出菇早,产量最高,单畦产量为5 319 g;21、19号和12号菌株相较于9号菌株出菇晚,单畦产量分别为4 341、4 059 g和3 429 g,经3个生产周期多点试验栽培,均取良好效果,初步确认这4个菌株可在寒冷地区推广使用。Twenty-two production strains were used as materials to compare the mycelium growth in cPDA medium and cultivated medium.The growth of cPDA medium and cultivated medium was studied under the cold conditions and the‘autumn sowing and spring harvest’model.Cultivation tests were also used to compare different strains,in order to screen suitable production strains of Morchella esculentafor cultivation in cold areas.The results showed that 22strains were divided into 3groups,according to the growth in cPDA medium;while they were divided into 2groups,according to the growth of cultivated tests.Base on the cultivation test results,the fruiting bodies of strain No.9 formed earlier,and strain No.9had the highest yield,with a single border yield of 5319g.The fruiting bodies formation time of strain No.21,No.19and No.12performed later than strain No.9,the yield was 4341g,4059g and 3429g,respectively.Good results were obtained after three production cycles of multi-point test cultivation.It was preliminarily confirmed that four strains could be popularized and used in cold areas.

关 键 词：羊肚菌 生产菌株 菌丝生长 栽培 

分 类 号：S646.6[农业科学—蔬菜学]