谷氨酸棒杆菌脂肪酸合成酶fasA基因的异源表达对脂肪酸合成的影响  

Effect of heterologous expression of fasA gene of Corynebacterium glutamicum fatty acid synthase on fatty acid synthesis

作　　者：金晶晶 孙子羽 满都拉[1] 刘瑾 胡晨玥 陈忠军[1] JIN Jingjing;SUN Ziyu;MAN Dula;LIU Jin;HU Chenyue;CHEN Zhongjun(College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学食品科学与工程学院,呼和浩特010018

出　　处：《中国油脂》2023年第4期94-98,104,共6页China Oils and Fats

基　　金：国家自然科学基金(31860439)。

摘　　要：旨在为脂肪酸高产菌株的筛选和构建奠定理论基础,研究谷氨酸棒杆菌脂肪酸合成酶A(FasA)对大肠杆菌脂肪酸合成的影响,利用生物信息学软件预测分析了FasA的性质并构建过表达载体pXMJ19-fasA,转化至大肠杆菌进行表达,对表达诱导剂异丙基-β-D-硫代吡喃半乳糖苷(IPTG)浓度进行考察,并测定了异源表达后的脂肪酸组成。结果表明,重组菌株在IPTG浓度为1 mmol/L下诱导20 h,棕榈油酸和油酸产量分别达到1.735、2.325 mg/g,与对照菌株相比分别提高了53.95%和59.68%。研究结果说明FasA对大肠杆菌合成棕榈油酸和油酸有促进作用。In order to lay a theoretical foundation for screening and constructing fatty acid high-yielding strains, the effects of Corynebacterium glutamicum fatty acid synthase A (FasA) on the synthesis of fatty acids in E. coli were studied, the properties of FasA were predicted and analyzed using bioinformatics software, moreover the overexpression vector pXMJ19-fasA was constructed and transformed into E. coli for expression, the concentration of expression inducer isopropyl-β-D-thiogalactoside (IPTG) was investigated and the fatty acid composition after heterologous expression was determined.The results showed that when the recombinant strain was induced under the IPTG concentration of 1 mmol/L for 20 h,the yield of palmitoleic acid and oleic acid reached 1.735,2.325 mg/g ,which increased by 53.95% and 59.68% respectively compared with the control strain. These results indicate that FasA can promote the synthesis of palmitoleic acid and oleic acid by E. coli.

关 键 词：脂肪酸合成酶 谷氨酸棒杆菌 棕榈油酸 油酸 异源表达 

分 类 号：TE667[石油与天然气工程—油气加工工程] TQ920.1[轻工技术与工程—发酵工程]