一株嗜热脂肪芽孢杆菌的筛选及其产酶特性研究  

Screening of a Geobacillus stearothermophilus Strain and Study on Its Enzyme-Producing Characteristics

作　　者：张运祺 郑自强 邢浩博 应超 兰茂华 任志强 卫春会 ZHANG Yunqi;ZHENG Ziqiang;XING Haobo;YING Chao;LAN Maohua;REN Zhiqiang;WEI Chunhui(Liquor Making Bio-technology&Application Key Laboratory of Sichuan Province,Sichuan University of Science&Engineering,Yibin,Sichuan 644000;Liquor Making Biotechnology and Intelligent Manufacturing Key Laboratory of China Light Industry,Yibin,Sichuan 644000,China)

机构地区：[1]四川轻化工大学酿酒生物技术及应用四川省重点实验室,四川宜宾644000 [2]中国轻工业酿酒生物技术及智能制造重点实验室,四川宜宾644000

出　　处：《酿酒科技》2023年第5期78-84,共7页Liquor-Making Science & Technology

基　　金：五粮液集团公司产学研合作项目(CXY2019ZR009);四川轻化工大学泸州老窖研究生创新基金(LJCX2022-3);四川轻化工大学大学生创新创业训练计划项目(cx2021137)。

摘　　要：从高温大曲中分离得到了一株产淀粉酶和蛋白酶的嗜热芽孢杆菌ZJY-1,研究了菌株ZJY-1的生长特性和产酶特性,并应用于强化麸曲探究其价值。经鉴定分析,菌株ZJY-1为嗜热脂肪芽孢杆菌(Geobacillus stearothermophilus),其最适生长pH为7.0,有良好嗜热性,最适生长温度为55℃,且在65℃高温下仍生长良好;菌株酶活力检测结果表明,不同发酵时间菌株功能酶活力存在差异,淀粉酶活力最高达到了133.5 U/g±12.5 U/g,蛋白酶活力最高为175.4 U/g±4.8 U/g;通过制备强化麸曲试验表明,ZJY-1麸曲具备分泌蛋白酶和淀粉酶能力,生物量指标可达到5.7×10^(10)±2.8×10^(9) CFU/g。本研究丰富了大曲微生物菌种资源,为高温曲发展方向提供了参考价值。A strain ZJY-1 with amylase and protease producing ability was isolated from high-temperature Daqu.The growth characteristics and enzyme producing characteristics of strain ZJY-1 were analyzed,and its value in the production of fortified Fuqu was explored.Strain ZJY-1 was identified as Geobacillus stearothermophilus.Its optimum growth pH was 7.0,its optimum growth temperature was 55℃,and it grew well under the temperature of 65℃.The enzyme activity test results of strain ZJY-1 showed that its functional enzyme activity was different at different fermentation stages;the amylase activity reached 133.5±12.5 U/g,and the protease activity reached 175.4±4.8 U/g.Fortified Fuqu made with strain ZJY-1 had the ability to secrete protease and amylase,and the biomass index reached up to 5.7×10^(10)±2.8×10^(9) CFU/g.This study has enriched the microbial resources for Daqu making,and has provided reference for the development of high-temperature Fuqu.

关 键 词：高温大曲 嗜热芽孢杆菌 淀粉酶 蛋白酶 麸曲 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.1[轻工技术与工程—食品科学与工程]