利用青海弧菌Q67评估5种农药的毒性作用  

Toxicity Assessment of Five Pesticides Using Vibrio qinghaiensis sp.Q67

作　　者：彭悦 文春露 杨春杰[1] 蒋圣启 丁武[1] PENG Yue;WEN Chunlu;YANG Chunjie;JIANG Shengqi;DING Wu(College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《食品科学》2023年第9期15-24,共10页Food Science

基　　金：陕西省重点研发计划项目(2021NY-153);陕西省技术创新引导专项(2021QFY10-06)。

摘　　要：以青海弧菌Q67(Vibrio qinghaiensis sp.Q67)为指示生物,通过毒性单位和浓度加和模型评估抗蚜威(pirimicarb,PIR)、西维因(carbaryl,CAR)、草甘膦(glyphosate,GLY)、乐果(dimethoate,DIM)、乙酰甲胺磷(acephate,ACE)这5种常见农药的毒性效应。结果表明,5种农药的毒性大小依次为PIR>GLY>CAR>DIM>ACE。4组二元混合体系联合毒性呈拮抗作用,其余组合为加和或协同效应。应用均匀设计射线法设计的7条五元混合物射线中,4条射线呈先协同后拮抗的作用趋势,3条射线始终呈拮抗作用,且五元混合体系毒性与单一组分CAR的浓度比呈良好的正相关关系(r=0.947),综上,可建立线性模型对混合物毒性进行预测。本研究所建立的方法成本低、速度快、操作简单、便于携带,可作为一种潜在的农产品、食品农药残留快速检测的初筛方法。Vibrio qinghaiensis sp.Q67 was used as an indicator strain to study the toxic effects offive common pesticides,pirimicarb(PIR),carbaryl(CAR),glyphosate(GLY),dimethoate(DIM),and acephate(ACE).The toxicities were evaluated by toxic unit and concentration addition model.The results showed that the toxicity of the pesticides decreased in the following order:PIR>GLY>CAR>DIM>ACE.Four of the 10 binary mixtures of these five pesticides showed antagonistic toxic effects,while the remaining six showed additive or synergistic toxic effects.Among the seven five-component mixtures designed by uniform design ray,four showed synergistic and then antagonistic effects,while the remaining three showed a consistent antagonistic effect.The toxicity of the five-component mixtures was strongly positively correlated with the concentration of CAR(r=0.947).Based on this result,a linear model was established to predict the toxicity of pesticide mixtures.This method has the advantages of low cost,rapidity,simplicity and convenience,which can be used as a potential preliminary screening method for rapid detection of pesticide residues in agricultural products and foods.

关 键 词：青海弧菌 联合毒性 农药 急性毒性 快速检测 

分 类 号：TS207.7[轻工技术与工程—食品科学]