金耳类酵母型菌株分离与高产胞外多糖培养基优化  

Isolation of yeast form strain from Tremella aurantialba and optimization of medium for its exopolysaccharide production

作　　者：张璐 李翘楚[1,2] 王增利 丁强[1] 王鸿磊[1] ZHANG Lu;LI Qiaochu;WANG Zengli;DING Qiang;WANG Honglei(Yantai Institute of China Agricultural University,Yantai 264670,Shandong,China;College of Food Science&Nutrition Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]中国农业大学烟台研究院,山东烟台264670 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《浙江农业学报》2023年第5期1154-1160,共7页Acta Agriculturae Zhejiangensis

基　　金：山东省现代农业产业技术体系食用菌产业体系烟台综合试验站(SDAIT-07-14)。

摘　　要：为获得纯金耳多糖,采用担孢子弹射法分离获得金耳类酵母型菌株(EF145),通过ITS序列测定对分离的菌株进行鉴定,测定金耳多糖的抗氧化性,通过正交试验优化高产胞外多糖培养基配方。结果显示:EF145菌株的菌体呈卵圆形,大小约为(2.5~4.0)μm×(2.0~4.0)μm,出芽增殖;菌落乳白色、表面凸起、光滑、湿润;其ITS序列与金耳子实体小条带一致,经Blast比对确定该菌株为金耳(Tremella aurantialba);该菌株多糖的羟自由基清除率为57.67%,超氧阴离子清除率为51.73%,DPPH自由基清除率为17.43%。EF145菌株培养的最佳碳源、氮源和无机盐分别是蔗糖、酒石酸铵和磷酸二氮钾,EF-145菌株产胞外多糖最佳培养基配方为蔗糖40 g·L^(-1)、酒石酸铵7.5 g·L^(-1)、磷酸二氢钾4 g·L^(-1)、土豆浸粉3 g·L^(-1)、维生素B 10.2 g·L^(-1)。In order to obtain pure exopolysaccharide from Tremella aurantialba through liquid fermentation,the yeast form strain EF145 of T.aurantialba was isolated by basidiospore ejection method and identified by ITS method,the antioxidant activity of its exopolysaccharide was studied.Orthogonal test was employed to optimize the medium formula for high yield exopolysaccharide.The result showed that the shape of EF145 was oval with a size of(2.5-4.0)μm×(2.0-4.0)μm,which proliferated by budding and its colonies were milky white,convex,smooth and moist.ITS sequence was consistent with the small band of T.aurantialba fruiting body,which was identified as T.aurantialba by BLAST analysis.The scavenging rates of hydroxyl radical,superoxide anion and DPPH radical of polysaccharide of T.aurantialba were 57.67%,51.73%and 17.43%,respectively.The best carbon,nitrogen and inorganic salts of EF145 were sucrose,ammonium tartrate and potassium dihydrogen phosphate,respectively.The optimal medium formula for exopolysaccharide production was sucrose 40 g·L^(-1),ammonium tartrate 7.5 g·L^(-1),potassium dihydrogen phosphate 4 g·L^(-1),potato powder 3 g·L^(-1) and vitamin B 10.2 g·L^(-1).

关 键 词：金耳 类酵母型菌株 胞外多糖 培养基优化 

分 类 号：S646[农业科学—蔬菜学]