市售酸奶发酵剂乳杆菌的耐药性及耐药基因定位研究  

Prevalence of antibiotic resistance and location of resistance genes in Lactobacillus isolated from commercial yogurt

作　　者：王炜哲 翟征远[1] 孙二娜 王辰元 郭慧媛[1] 郝彦玲[1] WANG Weizhe;ZHAI Zhengyuan;SUN Erna;WANG Chenyuan;GUO Huiyuan;HAO Yanling(Key Laboratory of Functional Dairy,Co-constructed by Ministry of Education and Beijing Municipality,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;Mengniu Hi-tech Dairy Product Beijing Co.Ltd.,Beijing 101107,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]蒙牛高科乳制品(北京)有限责任公司,北京101107

出　　处：《中国乳品工业》2023年第5期21-24,共4页China Dairy Industry

基　　金：国家重点研发计划(2018YFC1604303);北京市通州区科技成果转化项目(KJ2021ZH003)。

摘　　要：为探究酸奶发酵剂乳酸杆菌的耐药性及其安全性,对26份市售酸奶及发酵剂样品分离的乳酸杆菌抗生素耐药性及耐药基因进行定位研究。药敏结果显示,分离得到的29株乳酸杆菌对万古霉素耐药率高达51.72%,对链霉素和卡那霉素的耐药率均为20.69%,对庆大霉素完全敏感;获得性耐药性中,乳酸杆菌对氯霉素耐药率高达24.14%,克林霉素和四环素耐药率均为10.34%,对氨苄西林和红霉素耐药率均为3.45%。通过对9株获得性耐药乳酸杆菌质粒进行普查,结果表明仅有2株耐药菌株携带质粒。进一步利用二代测序分别对基因组和质粒进行测序,经生物信息学分析明确耐药基因定位在基因组上,因此目前收集的市售酸奶发酵剂中的乳酸杆菌未携带含获得性耐药基因的质粒,较为安全。In order to investigate the antibiotic resistance and the safety of Lactobacillus in commercial yogurt,the Lactobacillus strains isolated from 26 commercial yogurt and starter samples were investigated for antibiotic resistance and the localization of resistance genes.Antimicrobial susceptibility tests showed that the antibiotic resistance rate of the isolated 29 Lactobacillus strains to vancomycin,streptomycin and kanamycin reached up to 51.72%,20.69%and 20.69%,respectively.All the 29 Lactobacillus strains are completely sensitive to gentamicin.Among the acquired antibiotic resistance,the resistance rate of 29 Lactobacillus strains to chloramphenicol,clindamycin,tetracycline,ampicillin and erythromycin was 24.14%,10.34%,10.34%and 3.45%,respectively.Plasmid profile showed that Lactiplantibacillus plantarum L19 and Limosilactobacillus reuteri L27 harbored plasmid.In order to locate the gene encoding acquired antibiotics resistance,the genome and plasmid were sequenced by next-generation sequencing,and bioinformatic analysis showed that the antibiotic resistance genes were located on the genome.In this study,plasmid with acquired antibiotic resistance gene was not identified in Lactobacillus in commercial,indicating that Lactobacillus isolated from the collected commercial yogurt and starter don’t have the potential risk for transferring antibiotic resistance gene.

关 键 词：酸奶 乳酸杆菌 耐药性 耐药基因定位 

分 类 号：TS201.3[轻工技术与工程—食品科学]