非靶向代谢组学研究3种树胶代谢轮廓差异  

Untargeted Metabolomic Analysis of Differential Metabolite Profiles of Three Gums

作　　者：刘佳 李勇[1,2,3] 木其尔 田菊[2,3] 张希平 余向阳 LIU Jia;LI Yong;MU Qi’er;TIAN Ju;ZHANG Xiping;YU Xiangyang(School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212001,China;Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base,Ministry of Science and Technology,Nanjing 210014,China;Institute of Food Safety and Nutrition,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212001 [2]省部共建国家重点实验室培育基地-江苏省食品质量安全重点实验室,江苏南京210014 [3]江苏省农业科学院农产品质量安全与营养研究所,江苏南京210014

出　　处：《食品科学》2023年第10期273-280,共8页Food Science

基　　金：现代农业产业技术体系建设专项(CARS-30-5-03);江苏现代农业产业技术体系建设专项(JATS[2021]446)。

摘　　要：分析阿拉伯胶、雪燕、桃胶的总酚含量和代谢轮廓差异。结果表明,不同树胶的总酚含量存在明显差异,其中桃胶总酚含量最高(19.93μmol/g),其次是阿拉伯胶(4.06μmol/g)和雪燕(1.36μmol/g)。基于代谢组学分析,发现3种树胶均富含小分子代谢物,但不同树胶小分子代谢物种类及丰度存在明显差异,其可能与流胶树的品种及流胶方式有关。桃胶中类黄酮物质含量丰度明显高于阿拉伯胶和雪燕,与总酚含量趋势一致;阿拉伯胶的氨基酸、有机酸含量丰度明显高于桃胶和雪燕;雪燕中脂类物质含量丰度明显高于阿拉伯胶和桃胶。不同树胶差异代谢物富集的代谢通路主要为类黄酮生物合成、异喹啉生物碱生物合成、氨基酸合成与代谢、亚油酸代谢等。本研究基于代谢组学揭示不同树胶代谢物组成及丰度差异,研究结果将为不同树胶品质分析及功能成分挖掘提供技术支撑。In this study,the differences in total phenol content and metabolite profile among arabic gum,karaya gum and peach gum were analyzed.The results showed that there was a significant difference in total phenol content among the three gums.Peach gum had the highest total phenol content(19.93μmol/g),followed by arabic gum(4.06μmol/g)and karaya gum(1.36μmol/g).Metabolomics analysis showed that all these gums were rich in small molecular metabolites,such as amino acids,organic acids,flavonoids,and lipids.However,there were significant differences in the species and abundance of small molecular metabolites among the gums,which may be associated with producer species and flowing method.The contents of flavonoids and total phenols in peach gum were significantly higher than those in arabic Gum and karaya gum.In addition,the contents of amino acids and organic acids in karaya gum and arabic gum were significantly higher than those in peach gum.The content of lipids in karaya gum was significantly higher than that in arabic gum and peach gum.The metabolic pathways of differential metabolites among the different gums mainly included the pathways of flavonoid biosynthesis,isoquinoline alkaloid biosynthesis,amino acid synthesis and metabolism,and linoleic acid metabolism.The results from our present study may provide technical support for gum quality analysis and the discovery of its functional components.

关 键 词：树胶 代谢组学 类黄酮 总酚 差异代谢物 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]