表面活性剂对球孢白僵菌生物合成D-HPPA的影响  

Effect of surfactants on D-HPPA biosynthesis by Beauveria bassiana

作　　者：彭继兰 刘宗求 陈思宇 王金华 罗昕 王永泽 PENG Ji-lan;LIU Zong-qiu;CHEN Si-yu;WANG Jin-hua;LUO Xin;WANG Yong-ze(Hubei University of Technology,School of Bioengineering and Food Science,Key Laboratory of Fermentation Engineering of Ministry of Education,Wuhan 430068,China)

机构地区：[1]湖北工业大学生物工程与食品学院,发酵工程教育部重点实验室,工业发酵省部共建协同创新中心,湖北武汉430068

出　　处：《生物技术》2023年第2期248-253,267,共7页Biotechnology

基　　金：湖北省教育厅重点项目(D20201403);企业合作项目(2020240);湖北工业大学博士启动基金(XJ2021010301)。

摘　　要：[目的]提高目的产物R-(+)-2-(4-羟基苯氧基)丙酸(D-HPPA)的产量,研究不同表面活性剂对球孢白僵菌生物合成D-HPPA的影响。[方法]在固态发酵、液态发酵和全细胞催化三种生物合成方式中,分别考察表面活性剂的种类、浓度以及添加时间对D-PPA转化率的影响。[结果]固态发酵中添加0.5%的斯盘-80,液态发酵及全细胞催化中添加2.5%的斯盘-80均取得较高的D-PPA转化率,分别为99.64%±0.47%、60.60%±0.85%和54.50%±0.69%。在固态发酵和液态发酵方式中,发酵48 h时添加表面活性剂结果均为最佳,且表面活性剂添加时机与D-PPA一致。[结论]在三种生物合成方式中添加斯盘-80均能提高D-PPA的转化率。[Objective]To improve the yield of the target product R-(+)-2-(4-hydroxy-phenoxy) propionic acid(D-HPPA),the effects of different surfactants on the biosynthesis of D-HPPA by Beauveria bassiana were studied.[Method]The effects of surfactant type,concentration and addition time on the conversion of D-PPA were investigated in three biosynthesis methods,solid-state fermentation,liquid fermentation and whole-cell catalysis.[Result]Adding 0.5% Span-80 to solid-state fermentation and adding 2.5% Span-80 to liquid fermentation and whole-cell catalysis achieved higher D-PPA conversion rates,99.64%±0.47%,60.60%±0.85 %and 54.50%±0.69% respectively.Adding surfactant at 48 h in solid-state fermentation and liquid fermentation led to the best conversion results,the adding time of surfactant was consistent with that of D-PPA.[Conclusion] The conversion of D-PPA can be improved by adding Span-80 in the three biosynthesis methods.

关 键 词：球孢白僵菌 表面活性剂 固态发酵 液态发酵 全细胞催化 R-(+)-2-(4-羟基苯氧基)丙酸 

分 类 号：Q939.97[生物学—微生物学]