基于甘蔗糖蜜的黑曲霉产β-Ffase酶的单因素条件研究  

Study on Single Factor Condition ofβ-Ffase Production by Aspergillus niger based on Sugarcane Molasses

作　　者：雷光鸿 樊红日[1] 李元 陈亮 李莉娇 尹丽[1] LEI Guanghong;FAN Hongri;LI Yuan;CHEN Liang;LI Lijiao;YIN Li(College of Light Chemical Engineering,Guangxi Vocational and Technical College,Nanning,Guangxi 530003;College of Light Industry and Food Engineering,Guangxi University,Nanning,Guangxi 530004)

机构地区：[1]广西工业职业技术学院轻工化工学院,广西南宁530003 [2]广西大学轻工与食品工程学院,广西南宁530004

出　　处：《甘蔗糖业》2023年第2期40-46,共7页Sugarcane and Canesugar

基　　金：国家糖料产业技术体系(CARS-170502);广西自然科学基金(2018GXNSFAA050143);桂工业院科研(Y2020KY004)。

摘　　要：为了探明在甘蔗糖蜜体系下黑曲霉产β-Ffase酶的变化规律,本研究开展单因素实验。实验结果表明,在糖蜜添加量15%、氮源酵母粉20g/L、pH5.5、孢子接种量10%、温度30℃的黑曲霉菌株产β-Ffase发酵条件下,培养48h后,β-Ffase的酶活为463.87U/mL,结合表面活性剂吐温-80,与相关文献提供的培养条件相比,提高1.6倍,细胞干重为11.77 g/L,提高1.48倍。In order to investigate the variation of Aspergillus niger enzyme production in the sugarcane molasses system,we used single factor optimization experiment in this study.The results showed that the optimum fermentation conditions forβ-Ffase production of Aspergillus niger strains were as follows:15%molasses,20 g/L nitrogenous yeast powder,pH 5.5,10%spore inoculation,and temperature 30℃.After 48 h culture,β-Ffase enzyme activity was 463.87 U/mL,combined with surfactant Tween-80,1.6 times higher than the culture conditions provided by the literature,and the cell dry weight was 11.77 g/L,1.48 times higher.

关 键 词：甘蔗糖蜜 低聚果糖 黑曲霉 β-Ffase酶 

分 类 号：TS249.3[轻工技术与工程—制糖工程]