微波辅助H_(2)O_(2)/V_(C)降解制备低分子量浒苔多糖的研究  

Preparation of Low-molecular-weight Enteromorpha Polysaccharides by Microwave-assisted Degradation with H_(2)O_(2)/V_(C)

作　　者：杜国丰[1] 尹梦琪 梁飞龙 姜宁 陈红漫[3] 矫继峰[1] 刘凤翊 DU Guofeng;YIN Mengqi;LIANG Feilong;JIANG Ning;CHEN Hongman;JIAO Jifeng;LIU Fengyi(College of Chemistry and Environmental Engineering,Yingkou Institute of Technology,Yingkou 115014,China;Basic Courses Teaching and Research Department,Yingkou Institute of Technology,Yingkou 115014,China;College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110866,China)

机构地区：[1]营口理工学院化学与环境工程学院,辽宁营口115014 [2]营口理工学院外语教研部,辽宁营口115014 [3]沈阳农业大学生物科学技术学院,辽宁沈阳110866

出　　处：《食品工业科技》2023年第12期37-44,共8页Science and Technology of Food Industry

基　　金：辽宁省自然科学基金区域创新联合基金计划“海洋藻类多糖的分离及构效关系研究”(2022-YKLH-13);营口理工学院大学生创新创业项目“海洋藻类植物浒苔低聚糖的制备”(X202214435038)。

摘　　要：为制备抗氧化活性较强的低分子量多糖,以辽宁营口沿海地区的浒苔为原料,采用微波辅助H_(2)O_(2)/V_(C)降解法制备低分子量浒苔多糖,利用DEAE-52、Sephadex G-100进行层析分离,对比浒苔多糖降解前后的理化性质与抗氧化活性,通过红外光谱法对降解后的浒苔多糖进行结构表征,利用薄层层析、高效液相色谱对分离到的多糖LEPⅢa组分进行单糖组成分析。结果表明,利用微波辅助H_(2)O_(2)/V_(C)降解可在较短时间(15 min)降解得到低分子量的浒苔多糖,经层析分离后,得到3种低分子量浒苔多糖LEPⅠ、LEPⅡ、LEPⅢa,分子量Mw分别为(23.6±0.5)、(24.6±0.6)、(22.9±0.5)kDa。与未降解的浒苔多糖相比,微波辅助H_(2)O_(2)/V_(C)降解得到的低分子量多糖的分子量、粘度均显著降低(P<0.05);抗氧化活性显著增强(P<0.05)。降解产物的分子量均在30 kDa以下,粘度在2 mPa.s以下;8 mg/mL LEPⅢa的总抗氧化能力由降解前的(315.2±10.3)U/mL增加到(661.8±18.3)U/mL,铁离子还原能力由降解前的51.3%±1.0%增加到83.4%±2.2%。LEPⅢa的红外光谱结果表明其为一种硫酸多糖,硫酸基含量14.6%±0.5%;薄层层析结合高效液相色谱分析表明LEPⅢa是一种由葡萄糖、鼠李糖、糖醛酸等组成的含硫酸基团低分子量多糖,D-(+)-甘露糖、L-(+)-鼠李糖、D-葡糖醛酸、D-(+)-葡萄糖的摩尔比为0.97:4.43:3.13:24.8。本研究表明,利用微波辅助H_(2)O_(2)/V_(C)降解制备低分子量浒苔多糖,能够明显改善多糖的理化性质,显著提高其抗氧化能力,制备的多糖可以作为功能性成分用于抗氧化相关的功能食品和保健品的开发。In order to prepare low-molecular-weight polysaccharides with high antioxidant activity,low-molecular-weight Enteromorpha polysaccharides were prepared by the method of microwave-assisted H_(2)O_(2)/V_(C)degradation using Entermorpha prolifera from the coastal area of Yingkou,Liaoning Province as raw materials.In the present thesis,DEAE-52 and Sephadex G-100 were used for chromatography separation and physicochemical properties and antioxidant activity of Enteromorpha polysaccharide were compared before and after degradation.Then structural characterization of the degraded Enteromorpha polysaccharide was obtained by infrared spectroscopy,and monosaccharide composition of LEPⅢa(obtained by separation)was analyzed by thin layer chromatography(TLC)and high performance liquid chromatography(HPLC).Results showed that low-molecular-weight Entermorpha polysaccharide could be prepared in a short time(15 min)by microwave-assisted degradation with H_(2)O_(2)/V_(C)and three kinds of low-molecular-weight Entermorpha polysaccharide LEPⅠ,LEPⅡand LEPⅢa with Mw being(23.6±0.5),(24.6±0.6)and(22.9±0.5)kDa respectively were obtained by chromatographic separation.Compared with untreated Enteromorpha polysaccharide,low-molecular-weight polysaccharide obtained by microwave-assisted H_(2)O_(2)/V_(C)degradation were significantly reduced(P<0.05).The antioxidant activity was significantly enhanced(P<0.05).Molecular weight of the degradation products was lower than 30 kDa,with viscosity lower than 2 mPa·s.The total antioxidant capacity of LEPⅢa with a concentration of 8 mg/mL increased from(315.2±10.3)U/mL to(661.8±18.3)U/mL,and reducing capacity of ferric ion increased from 51.3%±1.0%to 83.4%±2.2%after degradation.Infrared spectroscopy of LEPⅢa showed that LEPⅢa was a kind of sulphated polysaccharide with sulfate accounting for 14.6%±0.5%.TLC and HPLC analysis showed that LEPⅢa was a low-molecular-weight polysaccharide containing sulfuric acid group,composed of glucose,rhamnose,uronic acid,etc.,with molar ratio o

关 键 词：多糖 低分子量浒苔多糖 制备 抗氧化活性 纯化 表征 

分 类 号：TS201.2[轻工技术与工程—食品科学]