超声提取结合LC-AFS法测定婴幼儿辅助食品中的无机砷  

Determination of Inorganic Arsenic in Infant Food Supplement by Ultrasonic Extraction Coupled with LC-AFS

作　　者：王婷 江婷婷 朱小德 李娜 吴兰兰 何梦颖 李家顺 WANG Ting;JIANG Tingting;ZHU Xiaode;LI Na;WU Lanlan;HE Mengying;LI Jiashun(Anhui Zhongchuang Food Testing Co.,Ltd.,Wuhu 241000,China)

机构地区：[1]安徽中创食品检测有限公司,安徽芜湖241000

出　　处：《食品工业科技》2023年第12期337-343,共7页Science and Technology of Food Industry

摘　　要：基于超声辅助提取,建立了不同婴幼儿辅助食品中无机砷的液相色谱-原子荧光光谱联用检测方法。样品首先经超声提取、正己烷净化、过膜,经阴离子柱(PRP-X100,10μm,4.1 mm×250 mm)分离。再以15 mmol/L磷酸氢二铵溶液(pH=6.0)为流动相,等度洗脱后进入原子荧光仪检测。结果表明:在5~100 ng/mL浓度范围内,各形态砷线性关系良好,各组分相关系数在0.999以上。该方法检出限为0.010 mg/kg。三水平加标回收率范围为88.7%~96.3%,相对标准偏差(RSD)为1.8%~4.9%。该方法具有样品前处理过程简单、提取效率高、分析速度快、准确度高和重复性好。因此可以满足婴幼儿辅助食品中不同形态无机砷的检测要求。Based on ultrasound-assisted extraction,a method for determination of inorganic arsenic in various supplementary food for infants and young children was established.The samples were successively subjected to ultrasonic extraction,n-hexane purification,membrane filtration,and anion column(PRP-X 100,10μm,4.1 mm×250 mm)separation.Then,15 mmol/L diammonium hydrogen phosphate solution(pH=6.0)was used as the mobile phase.After isocratic elution,it was detected by atomic fluorescence spectrometer.The results showed that the linear relationship of arsenic forms was good in the concentration range of 5~100 ng/mL,with correlation coefficient of each component of above 0.999.The detection limit of the method was 0.010 mg/kg.The recoveries of inorganic arsenic at three spiked concentration levels ranged from 88.7%to 96.3%,and the relative standard deviation(RSD)was 1.8%to 4.9%.The method had the advantages of simple pretreatment,high extraction efficiency,fast analysis speed,high accuracy and good repeatability.Therefore,it could meet the detection requirements of different forms of inorganic arsenic in various supplementary food for infants and young children.

关 键 词：婴幼儿辅助食品 无机砷 液相色谱-原子荧光光谱 超声提取 等度洗脱 

分 类 号：TS207.5[轻工技术与工程—食品科学]