壳聚糖/乳酸链球菌素复合抗菌膜的制备及其性能  

Preparation and properties of chitosan/nisin composite antibacterial film

作　　者：胡瀚文 余雪健 李旭 陈喆 贾雪岩 唐雪[1] 徐钢春[2] 陆乃彦[1] HU Hanwen;YU Xuejian;LI Xu;CHEN Zhe;JIA Xueyan;TANG Xue;XU Gangchun;LU Naiyan(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]中国水产科学研究院淡水渔业研究中心,江苏无锡214081

出　　处：《食品与发酵工业》2023年第11期13-19,共7页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31871865);江苏省现代农业(河蟹)产业技术体系(JATS[2019]471);2021年江苏省高等教育教改研究立项项目(2021 JSJG058);教育部第二批新工科研究与实践项目(E-SPNL20202312);教育部“新农科”研究与改革实践项目;中央级公益性科研专项院级基本业务费项目(2020TD62)。

摘　　要：微生物污染导致的各类食源性疾病频繁爆发,不仅威胁公众健康,还造成重大的经济损失。将天然生物抗菌剂应用于食品包装中制备复合抗菌膜,可以阻止微生物繁殖,增加食品货架期。该文通过京尼平将抗菌肽乳酸链球菌素和壳聚糖交联制备复合抗菌膜,并对其物理性能、机械性能和抗菌性能进行表征。结果表明,在抗菌膜中,随着京尼平和壳聚糖质量比的增加,抗菌膜颜色加深,孔隙增大,孔隙数量增加;随着京尼平含量增加到5.00%,抗菌膜的溶胀率从(425.71±2.80)%下降至(134.79±10.38)%,拉伸强度先从(58.65±1.21)MPa增加至(144.42±1.71)MPa再下降至(67.68±5.34)MPa,断裂伸长率从(7.29±0.49)%降低至(2.89±0.41)%;另外,抗菌实验结果表明,与未交联抗菌肽的壳聚糖膜相比,该复合膜对金黄色葡萄球菌有较好的抗菌活性。Foodborne diseases caused by microbial pollution not only threaten public health,but also cause great economic losses.Natural biological antibacterial agents were applied in food packaging to prepare composite antibacterial film,which can prevent microbial reproduction and increase food shelf life.In this paper,nisin and chitosan were crosslinked by genipin to prepare composite antibacterial films,and its physical properties,mechanical properties,and antibacterial properties were characterized.The results showed that when the ratio of genipin and chitosan in the antibacterial film increased,the color of the antibacterial film deepened,the pores increased,and the number of pores increased.As the content of genipin increased to 5.00%,the swelling rate of the antibacterial film decreased from(425.71±2.80)%to(134.79±10.38)%,the tensile strength increased from(58.65±1.21)MPa to(144.42±1.71)MPa and then decreased to(67.68±5.34)MPa,the elongation at break decreased from(7.29±0.49)%to(2.89±0.41)%.In addition,the antibacterial experiment showed that the composite membrane had better antibacterial activity against Staphylococcus aureus than the chitosan membrane without cross-linked antimicrobial peptide.

关 键 词：壳聚糖 乳酸链球菌素 京尼平 复合膜 包装材料 

分 类 号：TS206.4[轻工技术与工程—食品科学]