玫瑰多酚-壳聚糖纳米粒的制备及其抗氧化、降血糖活性研究  

Preparation of rose polyphenol-chitosan nanoparticles and their antioxidation and hypoglycemic activity

作　　者：王晓艺 申学建 张大虎 薛冰 陆世海 崔建东 WANG Xiaoyi;SHEN Xuejian;ZHANG Dahu;XUE Bing;LU Shihai;CUI Jiandong(State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science and Technology,Tianjin 300457,China;Shandong Dashu Dafu Special Food Co.Ltd.,Heze 274511,China)

机构地区：[1]天津科技大学,省部共建食品营养与安全国家重点实验室,天津300457 [2]山东大树达孚特膳食品有限公司,山东菏泽274511

出　　处：《食品与发酵工业》2023年第11期62-69,共8页Food and Fermentation Industries

基　　金：天津市科技计划项目(20ZYJDJC00080);山东省重点研发计划项目(2021CXGC010509)。

摘　　要：该研究采用离子交联法将玫瑰多酚包埋在壳聚糖中制备成玫瑰多酚-壳聚糖纳米粒,以包封率为评价指标,单因素试验得到最佳制备工艺条件为壳聚糖与三聚磷酸钠质量比为2∶1,乙酸体积分数为0.5%,玫瑰多酚添加量为3 mg/g,包封率为94.85%,比优化前提高了4.43%,此时玫瑰多酚负载率为21.26%。稳定性实验表明,光照和空气条件下,在壳聚糖纳米粒中的玫瑰多酚比游离玫瑰多酚更稳定。小鼠灌胃实验表明,玫瑰多酚-壳聚糖纳米粒可以减缓小鼠体重下降,降低空腹血糖值,提高口服葡萄糖耐量;提高小鼠体内超氧化物歧化酶、谷胱甘肽过氧化物酶活性,降低丙二醛含量。结果说明玫瑰多酚-壳聚糖纳米粒具有较好的抗氧化和降血糖活性。制备的玫瑰多酚-壳聚糖纳米粒不仅能够增强玫瑰多酚的生物利用率和稳定性,还可以提高其应用价值,在食品、药品、化妆品等行业领域具有广泛的应用前景。To improve the stability of rose polyphenol,rose polyphenol-chitosan nanoparticles were synthesized by encapsulating rose polyphenol into chitosan by ion crosslinking method.Using encapsulation rate as the evaluation index,the optimal preparation conditions of single factor test were that the mass ratio of chitosan/sodium tripolyphosphate was 2∶1,the volume fraction of acetic acid was 0.5%,the dosage of rose polyphenol was 3 mg/g,under the optimized conditions,encapsulation rate of rose polyphenol reached 94.85%,which was 4.43%higher than before optimization,and rose polyphenol loading rate was 21.26%.Stability experiments showed that rose polyphenols in chitosan nanoparticles were more stable than rose polyphenols under light and air conditions.Results showed that rose polyphenol-chitosan nanoparticles could slow down weight loss,decrease fasting blood glucose,and improve oral glucose tolerance in mice.Furthermore,rose polyphenol-chitosan nanoparticles increased the activities of superoxide dismutase and glutathione peroxidase and decreased the content of malondialde-hyde in mice.These results indicated that the rose polyphenol-chitosan nanoparticles had good antioxidant and hypoglycemic activities.The prepared rose polyphenol-chitosan nanoparticles can not only enhance the bioavailability and stability of rose polyphenols,and also improve their application value,which has a wide application prospect in food,medicine,cosmetics,and other industries.

关 键 词：玫瑰 多酚 壳聚糖纳米粒 抗氧化 降血糖 小鼠 

分 类 号：TS201.2[轻工技术与工程—食品科学] TB383.1[轻工技术与工程—食品科学与工程]