壳聚糖处理对采后脐橙活性氧代谢和细胞膜透性的影响  

Effects of Chitosan Treatment on Reactive Oxygen Species Metabolism and Cell Membrane Permeability in Postharvest Navel Orange Fruit

作　　者：曾玲珍 邓礼艳 林育钊 林河通[1,2,3] 林艺芬[1,2,3] Zeng Lingzhen;Deng Liyan;Lin Yuzhao;Lin Hetong;Lin Yifen(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002;Institute of Postharvest Technology of Agricultural Products,Fujian Agriculture and Forestry University,Fuzhou 350002;Key Laboratory of Postharvest Biology of Subtropical Special Agricultural Products,Fujian Province University,Fuzhou 350002)

机构地区：[1]福建农林大学食品科学学院,福州350002 [2]福建农林大学农产品产后技术研究所,福州350002 [3]亚热带特色农产品采后生物学(福建农林大学)福建省高校重点实验室,福州350002

出　　处：《中国食品学报》2023年第5期291-300,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：福建省高校产学合作项目(2019N5005)。

摘　　要：为了开发一种提高采后脐橙果实耐贮性的安全、简便、低成本保鲜技术,阐明其可能的作用机理,将采后“纽荷尔”脐橙果实用壳聚糖(稀释800倍的卡多赞)浸泡5 min(以清水浸泡5 min为对照)并在相对湿度85%和温度(18±1)℃下贮藏,研究壳聚糖处理对脐橙果实贮藏期间活性氧代谢和细胞膜透性的影响。结果显示:与对照果实比较,壳聚糖处理能较好地保持“纽荷尔”脐橙果实的外观品质,有效减少果实腐烂,抑制脐橙细胞膜透性、超氧阴离子自由基、过氧化氢和丙二醛含量的上升,保持较高的超氧化物歧化酶、抗坏血酸过氧化物酶和过氧化氢酶活性,较高的还原型谷胱甘肽和还原型抗坏血酸含量及较高的还原能力和1,1-二苯基-2-三硝基苯肼自由基清除能力。结论:壳聚糖(稀释800倍的卡多赞)处理增强了采后“纽荷尔”脐橙果实耐贮性,与壳聚糖处理增强脐橙果实的活性氧清除能力,降低活性氧和丙二醛的产生与积累,减轻膜脂过氧化作用及较好地保持其细胞膜结构有关。In order to develop safe,convenient and low-cost storage technology to improve the storability of postharvest navel orange fruit,and elucidate its possible mechanism,the harvested‘Newhall’navel oranges were treated with the chitosan,that is Kadozan with dilution of 1∶800(VKadozan∶VKadozan+water),for 5 min,and the‘Newhall’navel oranges soaked in water for 5 min as the control,then the fruit were stored at 80%relative humidity and(18±1)℃.This work was studied the effects of chitosan treatment on the reactive oxygen species(ROS)metabolism and membrane permeability in harvested‘Newhall’navel orange fruit.The results exhibited that,compared to control‘Newhall’navel oranges,chitosan treatment could better maintain fruit appearance quality and effectively reduce fruit decay of'Newhall'navel oranges during postharvest storage.Additionally,chitosan treatment could effectively suppress the increased cell membrane permeability,the increased contents of superoxide free radical,hydrogen peroxide and malondialdehyde,retain higher activities of superoxide dismutase,ascorbate peroxidase and catalase,higher contents of glutathione and ascorbic acid,and higher levels of reducing power and 1,1-diphenyl-2-trinitrophenylhydrazine free radical scavenging ability in'Newhall'navel oranges during storage.Therefore,the treatment of chitosan,Kadozan with dilution of 1∶800(VKadozan∶VKadozan+water),could effectively enhance the storability of harvested‘Newhall’navel orange fruit,which was associated with chitosan treatment enhancing ROS-scavenging ability to reduce the production and accumulation of ROS and malondialdehyde,and then alleviate membrane lipids peroxidation and maintain the better cell membrane structure integrity in postharvest‘Newhall’navel orange fruit.

关 键 词：“纽荷尔”脐橙 壳聚糖 活性氧代谢 细胞膜透性 耐贮性 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]