西藏曲拉中优良乳酸菌的筛选与评价  

Screening and Evaluation of Lactic Acid Bacteria Strains from Tibetan Qula

作　　者：吕嘉伟 卢灏泽 刘振东 薛蓓 王妍凌 杨冬莹 张海鹏 张金萍 黄翠 薛洁 LÜJiawei;LU Haoze;LIU Zhendong;XUE Bei;WANG Yanling;YANG Dongying;ZHANG Haipeng;ZHANG Jinping;HUANG Cui;XUE Jie(College of Food Science,Tibet Agriculture and Animal Husbandry University,Nyingchi,Tibet 860000;China National Research Institute of Food&Fermentation Industries Co.Ltd.,Beijing 100015;International Joint Research Center of Quality and Safety of Alcoholic Beverages,Beijing 100015;Medical College,Xizang Minzu University,Xianyang,Shaanxi 712082;College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi,Xinjiang 830052,China)

机构地区：[1]西藏农牧学院食品科学学院,西藏林芝860000 [2]中国食品发酵工业研究院有限公司,北京100015 [3]国家酒类品质与安全国际联合研究中心,北京100015 [4]西藏民族大学医学院,陕西咸阳712082 [5]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052

出　　处：《酿酒科技》2023年第6期34-45,共12页Liquor-Making Science & Technology

基　　金：西藏自治区自然科学基金项目(XZ202001ZR0003G)。

摘　　要：西藏曲拉是西藏传统民族乳制品的典型代表,具有蛋白质含量高、贮藏时间长等优点,在我国众多传统乳制品中独具特色。研究利用MRS培养基对曲拉样品中的微生物进行了分离纯化,筛选得到42株乳酸菌,经16S rDNA鉴定,得到肠膜明串珠(L.mesenteroides)11株、假肠膜明串珠菌(L.pseudomes-enteroides)5株、肠膜明串珠菌右旋葡聚糖亚种(L.mesenteroides subsp.dextranicum)4株、粪肠球菌(E.fae-calis)10株,植物乳杆菌(L.plantarum)4株、乳酸片球菌(P.acidilactici)4株、布氏乳杆菌(L.buchneri)2株、类肠膜魏斯氏菌(W.paramesenteroides)2株;经过发酵试验与耐受性试验、抑菌性试验,筛选出3株具有优良特性的乳酸菌,本研究筛选的乳酸菌可应用于乳制品的生产。Tibetan Qula is a typical traditional Tibetan dairy product with high protein content and long storage time.In this study,42 lactic acid bacteria strains were isolated and purified from Qula by using MRS medium.16S rDNA identification results showed that there were 11 strains of L.mesenteroides,5 strains of L.pseudomesenteroides,4 strains of L.mesenteroides subsp.dextranicum,10 strains of E.faecalis,4 strains of L.plantarum,4 strains of P.acidilactici,2 strains of L.buchneri,and 2 strains of W.paramesenteroi-des.After the fermentation test,tolerance test and bacteriostatic test,3 strains with excellent characteristics were screened out.The lactic acid bacteria strains obtained in this research can be applied to the production of dairy products.

关 键 词：曲拉 菌种鉴定 乳酸菌 发酵试验 耐受性试验 抑菌性试验 

分 类 号：TS261.1[轻工技术与工程—发酵工程] TS252.54[轻工技术与工程—食品科学与工程]