一种副干酪乳杆菌快速鉴定方法的建立及应用  

Establishment and Application of a Rapid Identification Method for Lactobacillus paracasei

作　　者：孙林慧 禚惠荣 师文君 王康琪 张东立 侯少阳 SUN Linhui;ZHUO Huirong;SHI Wenjun;WANG Kangqi;ZHANG Dongli;HOU Shaoyang(College of Pharmacy,Heze University,Heze 274015,China;Shandong Bigtree Dreyfus Special Meals Food Co.,Ltd.,Heze 274015,China)

机构地区：[1]菏泽学院药学院,山东菏泽274015 [2]山东大树达孚特膳食品有限公司,山东菏泽274015

出　　处：《食品安全导刊》2023年第15期100-104,共5页China Food Safety Magazine

基　　金：山东省自然科学基金(ZR2022QB146);菏泽学院博士基金(XY21BS35)。

摘　　要：目的:基于肠杆菌基因间重复一致序列聚合酶链反应技术(Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction,ERIC-PCR),寻找并制备一对引物探针,灵敏、准确地从复杂生境中鉴定副干酪乳杆菌。方法:寻找副干酪乳杆菌HP-B1145的肠杆菌基因间重复一致序列(Enterobacterial Repetitive Intergenic Consensus,ERIC)最佳反应体系,对ERIC片段回收纯化得到特定条带及序列结果,据此设计引物,快速鉴定副干酪乳杆菌。结果:根据回收得到的副干酪乳杆菌HP-B1145 ERIC片段,设计出了一对引物探针(1145-S-F:5’-GCAGGATGCTGATTGTTAGCAG-3’;1145-S-R:5’-CTCCGACCAAAGCGACTATGAC-3’)。结论:根据引物特异性、准确性、普适性、含量限度实验得出,设计的引物能准确灵敏地从复杂生境中鉴定副干酪乳杆菌。Objective:To search for and prepare a pair of primer probes based on ERIC-PCR technology for sensitive and accurate identification of Lactobacillus paracasei from complex habitats.Method:To search for the optimal ERIC reaction system for Lactobacillus paracasei HP-B1145,and obtain specific bands and sequence results through the recovery and purification of ERIC fragments.Based on this,primers were designed to achieve rapid identification of Lactobacillus paracasei.Result:A pair of primer probes(1145-S-F:5’-GCAGGATGCTGATTGTTAGCAG-3’;1145-S-R:5’-CTCCGACCAAAGCGACTATGAC-3’)were designed based on the recovered HP-B1145 ERIC Fragment.Conclusion:Based on the comprehensive experiments of primer specificity,accuracy,universality,and content limit,the designed primers can accurately and sensitively identify Lactobacillus paracasei from complex habitats.

关 键 词：副干酪乳杆菌 肠杆菌基因间重复一致序列聚合酶链反应技术(ERIC-PCR) 引物探针 特异性 鉴别 

分 类 号：TS201.3[轻工技术与工程—食品科学]