高效液相色谱法测定烧烤食品中苯并芘含量  

Determination of Benzopyrene Content in Barbecue Food by High Performance Liquid Chromatography

作　　者：李秋雨 LI Qiuyu(Guizhou Testing Technology Research and Application Center,Guiyang 550014,China)

机构地区：[1]贵州检测技术研究应用中心,贵州贵阳550014

出　　处：《食品安全导刊》2023年第15期140-144,共5页China Food Safety Magazine

摘　　要：苯并芘是一种致癌物质,易在烧烤食品中出现。本文利用高效液相色谱法检测烧烤食品中的苯并芘含量,以正己烷为提取溶剂,提取15 min,选择流动相为乙腈+水=80+20,使用高效液相色谱仪检测。苯并芘的检出限为0.2μg·kg^(-1),定量限为0.5μg·kg^(-1)。本方法操作简单,回收率高,检测经济成本低,适合用于烧烤食品中苯并芘的测定。Benzopyrene is a carcinogen that is easily found in barbecue food.In this paper,the benzopyrene content in barbecue food was detected by HPLC.N-hexane was used as extraction solvent for 15 min.The mobile phase was acetonitrile+water=80+20.The detection limit and quantitation limit of benzopyrene were 0.2μg·kg^(-1) and 0.5μg·kg^(-1),respectively.This method has the advantages of simple operation,high recovery and low cost,and is suitable for the determination of benzopyrene in barbecue food.

关 键 词：苯并芘 烧烤食品 回收率 固相萃取柱 高效液相色谱仪 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]