油酸对灵芝液态深层发酵合成羊毛甾醇代谢流分布的影响  

Effects of oleic acid on metabolic flux distributions of lanosterol synthesized by liquid submerged fermentation of Ganoderma lingzhi

作　　者：冯杰[1] 刘艳芳[1] 颜梦秋[1] 唐传红[1] 周帅[1] 张劲松[1] FENG Jie;LIU Yanfang;YAN Mengqiu;TANG Chuanhong;ZHOU Shuai;ZHANG Jingsong(Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs of the People's Republic of China,National Engineering Research Center of Edible Fungi,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]上海市农业科学院食用菌研究所,国家食用菌工程技术研究中心,农业农村部南方食用菌资源利用重点实验室,上海市农业遗传育种重点实验室,上海201403

出　　处：《菌物学报》2023年第6期1386-1395,共10页Mycosystema

基　　金：国家自然科学基金青年基金(31801919);上海人才发展资金资助项目(2019052);上海市农业科学院攀高计划(2022–2024年度);上海市农业科学院卓越团队建设计划(2022A-03)。

摘　　要：通过代谢通量方法在3L搅拌式发酵罐上研究了油酸对灵芝液态深层发酵代谢终产物羊毛甾醇代谢流分布的影响。结果表明,在灵芝的液态深层发酵过程中,添加油酸使合成羊毛甾醇的代谢通量提高45.29%;副产物麦角甾醇的含量由3.84 mg/g降低至0.02 mg/g;羊毛甾醇合成途径(LP)、三羧酸循环(TCA)、磷酸戊糖途径(PP)和糖酵解途径(EMP)的代谢通量比对照组分别提高了29.09%、14.52%、12.22%和2.11%。因此,添加油酸能够加强灵芝液态深层发酵过程中的整体代谢流分布,减少副产物麦角甾醇的积累,提高羊毛甾醇的代谢流量。该研究为下一步灵芝液态深层发酵中灵芝三萜得率的提高奠定研究基础。The effects of oleic acid on the metabolic flux distribution of lanosterol,the final products of liquid submerged fermentation of Ganoderma lingzhi,were studied by metabolic flux method in a 3 L stirred fermentor.The results showed that during the submerged fermentation of G.lingzhi,the metabolic flux of lanosterol increased by 45.29%by adding oleic acid to the liquid substrate.The content of by-product ergosterol decreased from 3.84 mg/g to 0.02 mg/g.The metabolic fluxes of lanosterol synthesis pathway(LP),tricarboxylic acid cycle(TCA),pentose phosphate pathway(PP)and glycolysis pathway(EMP)were increased by 29.09%,14.52%,12.22%and 2.11%as compared with those of the controls,respectively.Therefore,the addition of oleic acid could strengthen the overall metabolic flow distribution during submerged fermentation of G.lingzhi,reduce the accumulation of ergosterol,and improve the metabolic flow of lanosterol.This study laid a foundation for the improvement of triterpenoid yield in submerged fermentation of G.lingzhi.

关 键 词：灵芝 液态深层发酵 油酸 代谢流分析 羊毛甾醇 麦角甾醇 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]