锁掷孢酵母胞外多糖体外抗氧化和调节糖脂代谢的研究  

Antioxidant in vitro and glucolipid metabolism regulation of exopolysaccharides from Sporidiobolus pararoseus

作　　者：朱金峰 裴芳艺 刘振艳 孔婉莹 陈晓婷 ZHU Jinfeng;PEI Fangyi;LIU Zhenyan;KONG Wanying;CHEN Xiaoting(Office of Academic Research,Qiqihar Medical University,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔医学院科研处,黑龙江齐齐哈尔161006

出　　处：《中国酿造》2023年第6期109-115,共7页China Brewing

基　　金：黑龙江省教育厅项目(2022-KYYWF-0797)。

摘　　要：以近粉红锁掷孢酵母(Sporidiobolus pararoseus)PFY49为出发菌株,探究近粉红锁掷孢酵母PFY49产胞外多糖(EPS)的体外抗氧化、降血糖和胆汁酸结合能力,以及分析生物活性间的相关性。结果表明,近粉红锁掷孢酵母PFY49 EPS具有很好的持水性、水溶性、乳化性、热稳定性。同时具有良好的抗氧化活性,且呈剂量依赖性,对1,1-二苯基-2-三硝基苯肼(DPPH)、2,2’-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)、羟基自由基及亚硝基(NO2-)最大清除能力分别为(13.62±0.62)%、(66.82±0.61)%、(19.40±0.14)%、(67.45±0.36)%,总还原力(TRP)为0.33±0.01。近粉红锁掷孢酵母PFY49 EPS对α-淀粉酶和α-葡萄糖苷酶的最大抑制率分别为(51.47±0.84)%和(67.02±1.14)%,并且具有较好的体外胆汁酸结合能力。Using Sporidiobolus pararoseus PFY49 as the starting strain,the antioxidant in vitro,hypoglycemic and bile acid binding abilities of extracellular polysaccharide(EPS)produced by S.pararoseus PFY49 were investigated,and the correlation between biological activities was analyzed.The results showed that S.pararoseus PFY49 EPS had good water retention,water solubility,emulsification and heat stability.It also had good antioxidant activity in a dose-dependent manner.The maximum scavenging rates of 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)diamiammonium salt(ABTS),hydroxyl radical and nitroso were(13.62±0.62)%,(66.82±0.61)%,(19.40±0.14)%,(67.45±0.36)%,and the total reducing power(TRP)was 0.33±0.01.Maximum inhibition rates of S.pararoseus PFY49 EPS onα-amylase andα-glucosidase were(51.47±0.84)%and(67.02±1.14)%,respectively,and had better bile acid binding ability in vitro.

关 键 词：近粉红锁掷孢酵母 胞外多糖 生物活性 相关性分析 

分 类 号：TS201.3[轻工技术与工程—食品科学]