不同时期及不同区域汾酒大曲微生物群落结构分析研究  

Analysis of microbial community structure of Fenjiu Daqu in different periods and regions

作　　者：甄攀 韩英 王军燕 史斌斌 ZHEN Pan;HAN Ying;WANG Junyan;SHI Binbin(Shanxi Xinghuacun Fenjiu Distillery Co.Ltd.,Lvliang 032205,China)

机构地区：[1]山西杏花村汾酒厂股份有限公司,山西吕梁032205

出　　处：《中国酿造》2023年第6期140-145,共6页China Brewing

基　　金：山西杏花村汾酒厂股份有限公司重点研发计划(2021-B-YF-149)。

摘　　要：利用高通量测序分析了汾酒伏曲、冬曲等不同时期及不同区域生产用曲中细菌和真菌的组成结构及其差异。结果表明,共获得850个细菌操作分类单元(OTU)和223个真菌OTU,其中,包含54个细菌核心OTU和15个真菌核心OTU。不同大曲中的优势微生物组成相似,优势细菌属为泛菌属(Pantoea)、肠杆菌属(Enterobacter)、乳杆菌属(Lactobacillus)、葡萄球菌属(Staphylococcus)、芽孢杆菌属(Bacillus)、魏斯氏菌属(Weisella)等,优势真菌属为毕赤酵母属(Pichia)和覆膜孢酵母属(Saccharomycopsis)。但这些优势微生物在不同大曲中的丰度差异较大,尤其是伏曲,特点明显,拥有最高丰度的乳杆菌属及毕赤酵母属,为汾酒的优质稳定高产奠定了生物基础。Composition structure and differences of bacteria and fungi of Fenjiu Fu-qu and Dong-qu in different periods and regions were analyzed by high-throughput sequencing.The results showed that a total of 850 bacterial operational taxonomic units(OTU)and 233 fungal OTU were obtained,among them,including 54 bacterial core OTU and 15 fungal core OTU.The composition of dominant microorganisms in different Daqu was similar,the dominant bacteria genera were Pantoea,Enterobacter,Lactobacillus,Staphylococcus,Bacillus,and Weisella,etc.,and the dominant fungi genera were Pichia and Saccharomycopsis.However,the abundance of these dominant microorganisms varied greatly in the different Daqu,especially Fu-qu with obvious characteristics,which had the highest abundance of Lactobacillus and Pichia,laying biological foundation for the high quality,stability and high yield of Fenjiu.

关 键 词：清香型大曲 高通量测序 细菌群落 真菌群落 

分 类 号：TS261.3[轻工技术与工程—发酵工程]