GC-MS法分析银杏嫩叶的香气成分  

Analysis of aroma components of Ginkgo biloba tender leaves by GC-MS

作　　者：夏海越 李涛[1] 牟玉侦 XIA Haiyue;LI Tao;MOU Yuzhen(West China School of Pharmacy,Sichuan University,Chengdu,Sichuan,610041 P.R.China)

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2023年第3期294-296,共3页West China Journal of Pharmaceutical Sciences

基　　金：四川大学-达州市人民政府战略合作专项资金项目(2021CDDZ-22);四川省中医药管理局全国第四次中药资源普查科技专项项目(2019PC003)。

摘　　要：目的测定银杏嫩叶中香气成分的组成和相对含量。方法利用顶空固相微萃取技术萃取银杏嫩叶中的化学成分,并结合气相色谱-质谱联用法分析银杏嫩叶中香气化学成分的组成和含量。结果从银杏嫩叶的香气成分中解析出9种醇类、8种烃类及其衍生物、7种酯类、2种酮类、2种芳香化合物、1种醛类,共计29种化学成分。其中,含量超过3%的化合物有4-己烯-1-醇(47.33%)、甲酸已酯(15.03%)、反式-2-己烯-1-醇(6.68%)、乙醇(6.02%)、4-羟基-3-己酮(5.38%)、薁(5.30%)、异硫氰酸苯乙酯(3.61%)等。结论所用方法具有无损样品、无需萃取溶剂、自动便捷、灵敏度高等优点,为银杏嫩叶药用植物资源的研究与开发提供了依据。OBJECTIVE To analyze the composition and relative content of aroma chemical components in the tender leaves of Ginkgo biloba.METHODS HS-SPME technology was used to extract the chemical components from the tender leaves of G.biloba and GC-MS was adopted to analyze the composition and content of the aroma chemical components.RESULTS A total of 29 aroma chemical compounds were isolated and identified from the tender leaves of G.biloba,including alcohols,hydrocarbons and their derivatives,esters,ketones,aromatic compounds and aldehydes.The components with a content exceading 3%were 4-hexen-1-ol(47.33%),hexyl formate(15.03%),trans-2-hexen-1-ol(6.68%),ethanol(6.02%),4-hydroxyhexan-3-one(5.38%),azulene(5.30%),and phenylethyl isothiocyanate(3.61%).CONCLUSION The method is antomatic and convenient which requies no need to destroy samples solvent or use extraction.This paper provides an experimental basis for the research and development of the tender leaves of G.biloba.

关 键 词：银杏 嫩叶 顶空固相微萃取技术 气质联用技术 银杏叶茶 香气成分 4-己烯-1-醇 甲酸已酯 

分 类 号：R917[医药卫生—药物分析学]