非水相脂肪酶催化合成阿魏酸香茅醇酯  

Lipase catalyzed synthesis of citronellol ferulate in non-aqueous system

作　　者：陈林林[1] 王玲 杨茜瑶 宋佳琪 辛嘉英[1,2] CHEN Lin-lin;WANG Ling;YANG Xi-yao;SONG Jia-qi;XIN Jia-ying(Food Engineering College,Harbin University of Commerce,Harbin 150028,China;State Key Laboratory of Oxo Synthesis&Selective Oxidation Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China)

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150028 [2]中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室,甘肃兰州730000

出　　处：《化学研究与应用》2023年第6期1307-1315,共9页Chemical Research and Application

基　　金：黑龙江省自然科学基金项目(LH2020C063)资助;黑龙江省“百千万”工程科技重大专项项目(SC2021ZX04B0019)资助。

摘　　要：以固定化脂肪酶Novozyme435在非水相体系催化阿魏酸与香茅醇进行酯化反应,合成阿魏酸香茅醇酯。在对有机溶剂、脂肪酶加入量、反应温度、底物摩尔比等单因素实验考察的基础上,通过响应面实验对酶促合成阿魏酸香茅醇酯工艺进行优化。结果表明在反应体系为3 mL异辛烷中,阿魏酸与香茅醇的底物摩尔比为1∶6(其中阿魏酸加入量为0.5 mmol),脂肪酶加入量为105 g,分子筛加入量为0.5 g,65℃反应120 h后,酯化反应转化率可达90.39%,反应产率明显提高。The immobilized lipase novozyme435 was used to catalyze the esterification of ferulic acid and citronellol in a non-aqueous system to synthesize citronellyl ferulate.On the basis of single-factor experiments,organic solvents,the amount of lipase,reaction temperature,and molar ratio of substrate were examined,the enzymatic synthesis of citronellyl ferulate was optimized by response surface methodology.The results showed that in a reaction system of 3 mL isooctane,when ferulic acid and substrate molar ratio of citronellol 1∶6(wherein the amount of ferulic acid was 0.5 mmol),the amount of lipase was 105 g,and the amount of molecular sieve was 0.5 g,under 65℃after 120 h of the reaction,the esterification conversion rate could be up to 90.39%,which significantly improved the product yield of reaction.

关 键 词：阿魏酸 香茅醇 脂肪酶 酯化反应 非水相 

分 类 号：O621.4[理学—有机化学]