甘露低聚糖和部分水解瓜尔胶对小鼠慢性酒精性肝损伤的保护作用  

Protective Effects of Mannan Oligosaccharides and Partially Hydrolyzed Guar Gum on Chronic Alcoholic Liver Injury in Mice

作　　者：张荣彬 杨懿 周志磊[1,3,4] 姬中伟 任青兮 周建弟 徐岳正 毛健 ZHANG Rongbin;YANG Yi;ZHOU Zhilei;JI Zhongwei;REN Qingxi;ZHOU Jiandi;XU Yuezheng;MAO Jian(National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing,Jiangnan University,Wuxi 214122,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Jiangsu Provincial Engineering Research Center for Bioactive Product Processing,Synergetic Innovation Center of Food Safety and Quality Control,Wuxi 214122,China;Jiangnan University(Shaoxing)Industrial Technology Research Institute,Shaoxing 312000,China;National Engineering Research Center for Huangjiu,Shaoxing 312000,China;Zhejiang Guyuelongshan Shaoxing Wine Co.,Ltd.,Shaoxing 312000,China;Zhejiang Shaoxing Huangjiu Industry Innovation Service Complex,Shaoxing 312000,China)

机构地区：[1]江南大学粮食发酵与食品生物制造国家工程研究中心,江苏无锡214122 [2]江南大学食品学院,江苏无锡214122 [3]江苏省生物活性制品加工工程技术研究中心,食品安全与质量控制协同创新中心,江苏无锡214122 [4]江南大学(绍兴)产业技术研究院,浙江绍兴312000 [5]国家黄酒工程技术研究中心,浙江绍兴312000 [6]浙江古越龙山绍兴酒股份有限公司,浙江绍兴312000 [7]浙江省绍兴黄酒产业创新服务综合体,浙江绍兴312000

出　　处：《食品科学》2023年第11期95-105,共11页Food Science

基　　金：“十四五”国家重点研发计划重点专项(2021YFD2100102-4);国家自然科学基金青年科学基金项目(32001828);国家自然科学基金重点项目(22138004)。

摘　　要：目的:研究甘露低聚糖(mannan oligosaccharides,MOS)和部分水解瓜尔胶(partially hydrolyzed guar gum,PHGG)对长期酒精摄入导致小鼠肝损伤的抑制作用。方法:小鼠随机分为空白对照(Ctrl)组、乙醇模型(EtOH)组、阳性药物对照(PC)组(水飞蓟素86 mg/(kg·d))、MOS干预组(2000 mg/(kg·d))和PHGG干预组(2000 mg/(kg·d)),采用Lieber-DeCarli慢性酒精性肝损伤(alcoholic liver disease,ALD)模型造模5周后,测定肝功能、氧化应激和炎症因子水平;苏木精-伊红染色和油红O染色观察肝组织病理学改变;实时荧光定量聚合酶链式反应和免疫荧光染色法分别检测回肠紧密连接蛋白(闭锁小带蛋白-1和闭合蛋白)的mRNA和蛋白表达水平;酶联免疫吸附测定定量检测肝脏内毒素脂多糖(lipopolysaccharides,LPS)含量;气相色谱-质谱定量分析粪便样品中乙酸、丙酸、异丁酸、丁酸、异戊酸、戊酸和己酸的含量。结果:与EtOH组相比,补充MOS和PHGG均能够显著改善长期乙醇暴露引起的肝脏脂肪变性、脂质蓄积、氧化应激和炎症反应,恢复肝功能和肠道屏障功能,抑制肠源性LPS渗漏,并提高肠道短链脂肪酸(short-chain fatty acids,SCFAs)含量。结论:瓜尔胶来源的MOS和PHGG可以通过增强小鼠肠道完整性和调节肠道SCFAs水平有效缓解小鼠慢性ALD。Objective:To investigate the protective effects of mannan oligosaccharide(MOS)and partially hydrolyzed guar gum(PHGG)on liver injury induced by long-term alcohol intake in mice.Methods:Mice were randomly divided into control(Ctrl),model(EtOH),positive control(PC,silymarin at 86 mg/(kg·d)),MOS intervention(2000 mg/(kg·d))and PHGG intervention(2000 mg/(kg·d))groups.A Lieber-DeCarli model of chronic alcoholic liver injury(ALD)was established in this study.After five weeks of feeding,the levels of liver function,oxidative stress and inflammatory factors were measured.Hematoxylin-eosin(HE)staining and oil red O(ORO)staining were used to observe hepatic histopathological alterations.The mRNA and protein expression levels of ileal tight junction proteins(zonula occludens-1 and occludin)were detected by real-time fluorescence quantitative polymerase chain reaction(qPCR)and immunofluorescence staining,respectively.The hepatic lipopolysaccharide(LPS)level was quantitatively determined by enzyme-linked immunosorbent assay(ELISA).Besides,the contents of acetic acid,propionic acid,isobutyric acid,butyric acid,isovaleric acid,valeric acid and caproic acid in fecal samples were quantitatively analyzed by gas chromatography-mass spectrometry(GC-MS).Results:Supplementation of MOS and PHGG could significantly attenuate hepatic steatosis,lipid accumulation,oxidative stress and inflammation induced by long-term ethanol exposure.Additionally,compared with the EtOH group,MOS and PHGG intake markedly improved liver function and intestinal barrier function,inhibited intestinal LPS leakage,and increased intestinal short-chain fatty acids(SCFAs)levels.Conclusion:Guar gum-derived MOS and PHGG could effectively alleviate chronic ALD in mice by enhancing intestinal integrity and regulating intestinal SCFA levels.

关 键 词：甘露低聚糖 部分水解瓜尔胶 慢性酒精性肝损伤 肠道屏障功能 短链脂肪酸 

分 类 号：TS201.4[轻工技术与工程—食品科学] R151.1[轻工技术与工程—食品科学与工程]