大兴安岭地区蓝果忍冬果实多酚鉴定及生物活性分析  

Identification and Bioactivity Analysis of Polyphenols in Blue Honeysuckle Fruit from Different Regions of the Greater Khingan Mountains

作　　者：张妍 孙娟[1,3] 包菲菲 邓源源 刘佩 霍俊伟 ZHANG Yan;SUN Juan;BAO Feifei;DENG Yuanyuan;LIU Pei;HUO Junwei(College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,China;Heilongjiang Green Food Science Research Institute,Harbin 150030,China;National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions,Harbin 150030,China;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学园艺园林学院,黑龙江哈尔滨150030 [2]黑龙江省绿色食品科学研究院,黑龙江哈尔滨150030 [3]寒地小浆果开发利用国家地方联合工程研究中心,黑龙江哈尔滨150030 [4]东北农业大学农业农村部东北地区园艺作物生物学与种质创制重点实验室,黑龙江哈尔滨150030

出　　处：《食品科学》2023年第12期225-234,共10页Food Science

基　　金：国家人社部高层次留学人才回国资助项目(GCCLX005);黑龙江省博士后特别资助项目(LBH-TZ2004);黑龙江省应用技术研究与开发计划项目(GA20B301);国家葡萄产业技术体系蓝靛果哈尔滨综合试验站项目(CARS-29-10)。

摘　　要：以大兴安岭6个地区(加格达奇、新林、宏伟、阿木尔、图强和塔河)的蓝果忍冬果实为研究对象,测定并比较其总酚含量及总花色苷含量的不同;使用3种不同抗氧化方法全面分析其抗氧化活性;通过测定其对α-淀粉酶和α-葡萄糖苷酶的半抑制活性评价其降血糖活性;对其果实多酚粗提液进行分离纯化,并对不同组分进行定性定量分析。结果表明,这6个地区的蓝果忍冬果实总酚含量和总花色苷含量有明显差异,但组成基本相同,均具有较高的抗氧化活性;对α-淀粉酶、α-葡萄糖苷酶有一定抑制效果。表明这6个地区蓝果忍冬果实有开发利用的潜能。In this study,the contents of total phenols and total anthocyanins in blue honeysuckle fruit from six regions(Jiagedaqi,Xinlin,Hongwei,Amur,Tuqiang and Tahe)of the Greater Khingan Mountains were determined and compared,the antioxidant capacity of phenols from blue honeysuckle fruit was comprehensively analyzed using three different methods,and the hypoglycemic activity was evaluated by measuring its half maximal inhibitory concentration(IC50)againstα-amylase andα-glucosidase.The crude extract rich in polyphenols were separated and purified,and the resulting fractions were analyzed qualitatively and quantitatively.The results showed that the contents of total phenols and total anthocyanins in blue honeysuckle fruit from these six regions differed significantly,but the compositions were basically the same and all phenolic extracts had high antioxidant capacity.They had inhibitory activity againstα-amylase andα-glucosidase,indicating the potential of blue honeysuckle fruit from these six regions for development and utilization.

关 键 词：蓝果忍冬 多酚 抗氧化活性 抗α-淀粉酶活性 抗α-葡萄糖苷酶活性 

分 类 号：S663.9[农业科学—果树学]