DEtail-seq is an ultra-efficient and convenient method for meiotic DNA break profiling in multiple organisms  

作　　者：Wei Xu Chao Liu Zhe Zhang Changbin Sun Qin Li Kuan Li Hui Jiang Wei Li Qianwen Sun 

机构地区：[1]School of Life Sciences,Tsinghua University,Beijing,100084,China [2]Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangzhou,510623,China [3]Department of Urology,Department of Andrology,Department of Reproductive Medicine Center,and Department of Human Sperm Bank,Peking University Third Hospital,Beijing,100191,China [4]Tsinghua-Peking Center for Life Sciences,Beijing,100084,China [5]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,518120,China

出　　处：《Science China(Life Sciences)》2023年第6期1392-1407,共16页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (91740105,31822028,32071437,31900302);Central Public-interest Scientific Institution Basal Research Fund (Y2022QC33)。

摘　　要：Programmed DNA double-strand break(DSB)formation is a crucial step in meiotic recombination,yet techniques for highefficiency and precise mapping of the 3’ends of DSBs are still in their infancy.Here,we report a novel technique,named DNA End tailing and sequencing(DEtail-seq),which can directly and ultra-efficiently characterize the 3’ends of meiotic DSBs with near single-nucleotide resolution in a variety of species,including yeast,mouse,and human.We find that the 3’ends of meiotic DSBs are stable without significant resection in budding yeast.Meiotic DSBs are strongly enriched in de novo H3K4me3 peaks in the mouse genome at leptotene stage.We also profile meiotic DSBs in human and find DSB hotspots are enriched near the common fragile sites during human meiosis,especially at CCCTC-binding factor(CTCF)-associated enhancers.Therefore,DEtail-seq provides a powerful method to detect DSB ends in various species,and our results provide new insights into the distribution and regulation of meiotic DSB hotspots.

关 键 词：MEIOSIS DSB DNA break fragile site ENHANCER SPERMATOGENESIS 

分 类 号：Q75[生物学—分子生物学]