生姜蛋白酶无机大分子杂化纳米花的制备与研究  

Preparation and Study of Ginger Protease Inorganic Macromolecule Hybrid Nanoflower

作　　者：罗璇 陈玥 雷晶晶 马才学 沈瑞敏 LUO Xuan;CHEN Yue;LEI Jing-jing;MA Cai-xue;SHEN Rui-min(School of Food and Biotechnology,Wuhan Institute of Design and Sciences,Wuhan 430205,China)

机构地区：[1]武汉设计工程学院食品与生物科技学院,武汉430205

出　　处：《中国调味品》2023年第7期81-87,共7页China Condiment

基　　金：湖北省教育厅科研项目(B2021379);国家级大学生科技创新创业项目(201814035006);武汉设计工程学院科研项目(K201911)。

摘　　要：为提高游离生姜蛋白酶的酶活力,文章利用生姜蛋白酶和无机金属盐离子通过组装的方法成功合成了一种具有多级花状结构的新型纳米材料。通过扫描电子显微镜确定了生姜蛋白酶-无机杂化纳米花的组成和结构,并进一步优化了生姜蛋白酶无机大分子杂化纳米花的制备条件,研究了其酶学性质和使用稳定性,初步探索了纳米花酶活力与其形貌结构的关系。实验结果表明,以生姜蛋白酶为有机成分,磷酸铜为无机成分,制备的酶-无机杂化纳米花的形貌结构与酶浓度、Cu^(2+)浓度、pH、温度等因素密切相关,纳米花外表圆润、花瓣饱满、形状规整、粒径相似时,生姜蛋白酶-无机杂化纳米花的酶活也更高。当酶浓度为0.5 mg/mL、CuSO_(4)浓度为0.8 mol/L、pH为6.0、温度为4℃时,酶活力最高,可达到10302.2 U/mg,是游离生姜蛋白酶的7000%,温度、酸碱稳定性较高,重复使用性较好。To improve the enzyme ability of free ginger protease,in this paper,a new type of nano material with multistage floral structure is successfully synthesized by assembly of ginger protease and inorganic metal salt ions.The composition and structure of ginger protease-inorganic hybrid nanoflower are determined by scanning electron microscopy,the preparation conditions of ginger protease inorganic macromolecule hybrid nanoflower are further optimized,the enzymatic properties and use stability are studied,and the relationship between nanoflower enzyme activity and its morphological structure is preliminarily explored.Experimental results show that with ginger protease as the organic component,copper phosphate as the inorganic component,the morphological structure of the prepared enzyme-inorganic hybrid nanoflower is closely related to enzyme concentration,Cu^(2+)concentration,pH,temperature and other factors.When nanoflower has round appearance,plump petals,regular shape and similar particle size,ginger protease-inorganic hybrid nanoflower enzyme activity is higher.When the enzyme concentration is 0.5 mg/mL,the CuSO_(4) concentration is 0.8 mol/L,the pH is 6.0,and the temperature is 4℃,the enzyme ability is the highest,which can reach 10302.2 U/mg,7000%of free ginger protease enzyme activity.The temperature and acid-base stability is higher,and the reusability is better.

关 键 词：生姜蛋白酶 无机杂化纳米花 形貌结构 固定化酶 

分 类 号：TS201.25[轻工技术与工程—食品科学]