Study on the mechanism of the anti-tumor effects of matrine via regulation of lipid metabolism mediated by SREBP signaling pathway  

作　　者：Fang-Hua Wu Fei Huang Yan Chen Kui Chen Chao Gong Shen-Zhou Huang Jiang-Yue Yu Ruo-Qin Zhao Pei-Wen Zhu Li-Qun Wang 

机构地区：[1]Surgery Department,Affiliated Fuzhou First Hospital of Fujian Medical University Fujian 350009,China [2]Forestry Institute,Shanxi Agricultural University,Shanxi 030801,China

出　　处：《Cancer Advances》2023年第12期1-5,共5页TMR肿瘤进展

基　　金：This work was supported by grants from the Natural Science Foundation of Fujian province(No.2020J011181).

摘　　要：Objective:To investigate the therapeutic effects of matrine on CT26 tumor-bearing mice and its effect on the sterol regulatory element binding protein(SREBP)signaling pathway.Methods:A CT26 tumor-bearing mouse model was established using CT26 cells.Different doses of matrine were orally administered to mice,and the tumor size and weight in each group of mice before and after administration were measured to calculate the tumor inhibition rate of matrine.Subsequently,tumor tissues were subjected to hematoxylin and eosin(HE)staining to observe morphological changes in tumor tissue,and quantitative polymerase chain reaction(qPCR)was performed to detect the expression of the genes of the lipid metabolism-related enzymes sterol regulatory element binding transcription factor 1(Srebf1),ATP citrate lyase(Acly),acetyl-Coenzyme A carboxylase alpha(Acc),and fatty acid synthase(Fasn)in tumor tissues before and after matrine intervention.Results:Compared to those in the model group,tumor-bearing mice in both the low and high-dose matrine groups showed significantly reduced tumor weights.HE staining showed that matrine significantly inhibited tumor cell proliferation in both the low and high-dose groups.The qPCR results showed that,compared with the model group,the expression levels of the genes of lipid metabolism-related enzymes Srebf1,Acly,Acc,and Fasn in tumor tissues were significantly downregulated in both the low and high-dose matrine groups.Conclusion:Matrine modulates the lipid metabolism pathway,affects tumor cell lipid metabolism,and exerts antitumor effects.

关 键 词：MATRINE CT26 TUMOR lipid metabolism SREBP 

分 类 号：R73[医药卫生—肿瘤]