FAM136A调控胃癌进展的分子机制研究  

The Molecular Mechanism of FAM136A Regulating the Progression of Stomach Carcinoma

作　　者：付星玮 李晓波 耿飞翔 薄威[1] 王旭光[1] 张忠[1] 张珉[1] FU Xingwei;LI Xiaobo;GENG Feixiang;BO Wei;WANG Xuguang;ZHANG Zhong;ZHANG Min(Department of Pathology,Basic Medical College,Shenyang Medical College,Shenyang 110034,China)

机构地区：[1]沈阳医学院基础医学院病理教研室,辽宁沈阳110034

出　　处：《中国医学创新》2023年第18期56-61,共6页Medical Innovation of China

基　　金：2022年辽宁省自然科学基金计划项目(2022-NLTS-14-04);2019年辽宁省教育厅一般项目(310170007);沈阳医学院硕士研究生科技创新基金项目(Y20210504)。

摘　　要：目的:研究FAM136A(family with sequence similarity 136,member A gene)在胃癌进展中的调控作用。方法:通过生信分析筛选出FAM136A在胃癌中转录水平,通过免疫组织化学染色技术分析FAM136A在胃癌组织中表达特点及临床病理意义,通过侵袭实验(Transwell实验)、细胞活力检测实验(MTT实验)检测FAM136A对胃癌细胞迁移、侵袭、增殖能力的影响。结果:FAM136A在胃癌中转录水平明显高于癌旁组织,FAM136A在胃癌组织中高表达,主要定位于细胞质,病理分期Ⅱ、Ⅲ期、T分期T2~4、N分期1~3期的高表达比例高于病理分期Ⅰ期、T分期T1及N分期0期(P<0.01)。FAM136A增强胃癌细胞迁移、侵袭和增殖能力。FAM136A增强胃癌细胞中上皮间质转化(EMT)和细胞周期相关蛋白的表达。结论:FAM136A可通过调控EMT增强胃癌细胞迁移和侵袭能力。FAM136A可通过调节细胞周期促进胃癌增殖能力。Objective:To study the regulation of FAM136A(family with sequence similarity 136,member A gene)in gastric cancer progression.Method:Bioinformatics analysis was used to screen the transcriptional level of FAM136A in gastric cancer,immunohistochemical staining was used to analyze the expression characteristics of FAM136A in gastric cancer tissues and clinicopathological significance.Transwell and MTT assays were used to detect the effects of FAM136A on the migration,invasion and proliferation of gastric cancer cells.Result:The transcription level of FAM136A in gastric cancer was significantly higher than that in adjacent gastric cancer,FAM136A was highly expressed in gastric cancer tissues,mainly located in the cytoplasm,the proportion of high expression in pathological stagesⅡ,Ⅲ,T stage T2-4,and N stage 1-3 were higher than those in pathological stagesⅠ,T stage T1,and N stage 0(P<0.01).FAM136A enhanced gastric cancer cell migration,invasion and proliferation.FAM136A enhanced EMT and the expression of cell cycle-related proteins in gastric cancer cells.Conclusion:FAM136A can enhance the migration and invasion ability of gastric cancer cells by regulating EMT.FAM136A can promote gastric cancer proliferation ability by regulating cell cycle.

关 键 词：FAM136A 胃癌 肿瘤进展 

分 类 号：R735.2[医药卫生—肿瘤]