Peptide WCPFSRSF ameliorates excitotoxicity and elevates synaptic plasticity in glutamate-damaged SH-SY5Y cells by modulating the PI3K/mTOR/EIF4E and BDNF/CREB/TrkB pathways  

作　　者：Qi Zhang Lin Zheng Guowan Su Donghui Luo Mingtao Huang Yunzi Feng Mouming Zhao 

机构地区：[1]School of Food Science and Engineering,South China University of Technology,Guangzhou,510640,China [2]Chaozhou Branch of Chemistry and Chemical Engineering Guangdong Laboratory,Chaozhou,521000,China [3]Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center,Guangzhou,510650,China

出　　处：《Food Bioscience》2022年第3期819-828,共10页食品生物科学（英文）

基　　金：financially supported by the National Key R&D Program of China(2018YFD0901102);Guangdong Provincial Key R&D Program(2020B020226005);the Specific Fund Program for Basic and Applied Basic Research of Guangdong Province(2019A1515011952);Shandong Provincial Key R&D Program(LJNY202018).

摘　　要：The purpose of this study was to illustrate the beneficial effects and underlying mechanisms of peptide Trp-Cys-Pro-Phe-Ser-Arg-Ser-Phe (WCPFSRSF) against excitotoxicity by transcriptome analysis combined with western blot in glutamate-treated neuronal cells.Our results demonstrated that WCPFSRSF restored the cell membrane damage induced by glutamate and promoted cell survival in SH-SY5Y cells.The RNA-sequencing analysis illustrated 376 genes (98 upregulated and 278 downregulated) were differentially expressed between the glutamate- and WCPFSRSF-treated cells.Furthermore,RNA-sequencing screening and further western blot results showed that WCPFSRSF regulated cAMP-related signaling and synapse-related function,which further promoted the translation and protein synthesis,followed by cell survival,neurotrophic pathway and long-term memory.The potential mechanisms of WCPFSRSF were related to the Akt/mTOR/EIF4E signaling pathway and CREB/TrkB/BDNF pathway.Taken together,our study demonstrated that WCPFSRSF could be used as a regulator for synaptic plasticity and neurotrophic-related pathway,which provided evidence that WCPFSRSF is a promising therapeutic strategy for neuroprotection and memory enhancement.

关 键 词：PEPTIDE EIF4E RNA-Sequencing analysis NEUROTROPHIN Synaptic plasticity 

分 类 号：TS201.4[轻工技术与工程—食品科学]