乳酸菌的基因组编辑技术研究进展  

Genome editing technology of lactic acid bacteria

作　　者：赵难难 贾爱卿 王永强 林影[3] 王健 ZHAO Nannan;JIA Aiqing;WANG Yongqiang;LIN Ying;WANG Jian(Animal Husbandry and Fisheries Research Center of Guangdong Haid Group Limited Company by Shares,Guangzhou 511400,Guangdong,China;Guangdong Haid Group Limited Company by Shares,Guangzhou 511400,Guangdong,China;School of Biology and Biological Engineering,South China University of Technology,Guangzhou 511400,Guangdong,China)

机构地区：[1]广东海大集团股份有限公司畜牧水产研究中心,广东广州511400 [2]广东海大集团股份有限公司,广东广州511400 [3]华南理工大学生物科学与工程学院,广东广州511400

出　　处：《微生物学通报》2023年第7期3088-3103,共16页Microbiology China

基　　金：广州市高水平企业研究院自主选题项目专题(202205110006)。

摘　　要：乳球菌(Lactococcus sp.)和乳杆菌(Lactobacillus sp.)是工业上常用的乳酸菌(lactic acid bacteria,LAB),长期应用于食品和饮料的发酵。近年来,随着分子操作及遗传改造技术的不断完善,推动了乳球菌和乳杆菌的基础和应用研究,其作为功能菌株和工业微生物细胞工厂的重要潜能也不断突显出来。本文综述了工业常用乳酸菌的基因组编辑技术研究进展,着重介绍了基于整合质粒的敲除、敲入,基于基因组重组工程的精细修饰和敲除、敲入,以及基于成簇的规律性间隔的短回文重复序列及其相关蛋白9[(clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated nuclease9(Cas9),(CRISPR/Cas9]系统的基因组编辑技术。Lactococcus sp.and Lactobacillus sp.are lactic acid bacteria commonly used in industry and have long been used in food and beverage fermentation.In recent years,with the continuous improvement of molecular manipulation and genetic modification techniques, the basic research and application of Lactococcus and Lactobacillus have been promoted, and their important potential as functional strains as well as industrial microbial cell factories has been highlighted. In this paper, the research progress of genome editing technology of lactic acid bacteria commonly used in industry was reviewed, and the knockout knock-in based on integrative plasmid, fine modification and knockout knock-in based on genome recombination engineering, and gene editing of CRIPSR/Cas9 system based on clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated nuclease9 (Cas9) were emphatically introduced.

关 键 词：乳球菌 乳杆菌 整合质粒 基因组重组工程 CRIPSR/Cas9系统 

分 类 号：TS201.3[轻工技术与工程—食品科学] Q78[轻工技术与工程—食品科学与工程]