油莎豆水溶性糖提取工艺及生物转化低聚果糖的研究  

Extraction Technology of Water-Soluble Sugar from Cyperus esculeutus and its Biotransformation into Fructooligosaccharides

作　　者：李晨飞 闫晓侠 董舒月 刁梦雪 卢笑雨 于亚莉 张铁华 Li Chenfei;Yan Xiaoxia;Dong Shuyue;Diao Mengxue;Lu Xiaoyu;Yu Yali;Zhang Tiehua(College of Food Science and Engineering,Jilin University,Changchun 130062)

机构地区：[1]吉林大学食品科学与工程学院,长春130062

出　　处：《中国粮油学报》2023年第6期127-132,共6页Journal of the Chinese Cereals and Oils Association

基　　金：吉林省油莎豆产业发展重大科技专项(ZKJCKJT2020485)。

摘　　要：以油莎豆粕为原料,分离碳水化合物后采用酶法制备低聚果糖。结果表明,超声时间、提取温度和提取时间对提取效果的影响均呈显著水平且影响程度依次递减。优化工艺条件经调整为超声时间16 min、提取温度75℃、提取时间3 h,在此优化条件下具有较高的提取率(52.61±0.51)%。纯化工艺创新性的使用了等电点沉淀法与膜分离技术相结合对油莎豆粕水溶性糖进行脱蛋白,蛋白质清除率达到了51.35%,糖保留率为64.91%。利用果糖基转移酶转化生产低聚果糖,质量分数达到30.25%。Carbohydrate was separated from Cyperus esculeutus meal and fructooligosaccharides were manufactured by enzymatic method.The influence of ultrasonic time,extraction temperature and extraction time was significant,and they were in descending order based on the analysis of variance.At 75℃,16 min sonication time and 3 h extraction time,the optimal conditions recorded an extraction yield of(52.61±0.51)%.Afterwards,an innovative deproteinization and purification of water-soluble sugar from Cyperus esculeutus meal was carried out by using isoelectric point precipitation method and membrane separation technology.The protein clearance rate reached 51.35%and the sugar retention rate was 64.91%.Fructooligosaccharides were converted by fructosyltransferase,and the transformation rate reached 30.25%.

关 键 词：油莎豆粕 膜分离技术 低聚果糖 果糖基转移酶 

分 类 号：TS209[轻工技术与工程—食品科学]