速溶芦笋粉的酶解工艺优化及其改善小鼠睡眠功能  

Optimization of Enzymatic Hydrolysis Process and Effect of Sleep-improvement on Mice of the Instant Asparagus Powder

作　　者：杨晓春[1,2] 黎重阳 张玲玲 王荣春 张英春[1] 卢卫红[1,2] YANG Xiaochun;LI Chongyang;ZHANG Lingling;WANG Rongchun;ZHANG Yingchun;LU Weihong(School of Medicine and Health Engineering,Harbin Institute of Technology,Harbin 150001,China;The Intelligent Equipment Research Center for the Exploitation of Characteristic Food&Medicine Resources,Chongqing Research Institute of HIT,Chongqing 401135,China;Sichuan Gongda Southwest Food Research Co.,Ltd.,Bazhong 636063,China)

机构地区：[1]哈尔滨工业大学医学与健康学院,黑龙江哈尔滨150001 [2]哈尔滨工业大学重庆研究院,特色药食资源开发智能化装备研究中心,重庆401135 [3]四川工大西南食品研究有限责任公司,四川巴中636063

出　　处：《食品工业科技》2023年第15期175-183,共9页Science and Technology of Food Industry

基　　金：四川省科技计划资助(2021YFSY0035,2022ZHXC0003);黑龙江“头雁”团队(HITTY-20190034)。

摘　　要：为研究速溶芦笋粉的酶解工艺条件及其改善小鼠睡眠功能,以恩阳芦笋为主要原料,速溶芦笋粉的出粉率和皂苷含量为指标,在将芦笋烫漂、打浆后,利用纤维素酶和果胶酶(按质量比1:1复配)对其进行酶解,以不同复合酶添加量、酶解温度、酶解时间、pH为实验因素,通过单因素实验和响应面试验确定最佳酶解工艺条件。同时,分别进行戊巴比妥钠阀下剂量催眠试验、睡眠潜伏期试验和延长睡眠时间试验,通过比较各组入睡动物数和睡眠发生率、睡眠潜伏期和睡眠时间,对其改善小鼠睡眠的功能进行了研究。结果表明:速溶芦笋粉的最优酶解条件为复合酶添加量0.12%、酶解温度68℃、酶解时间2.2 h、酶解pH5.2,在此条件下速溶芦笋粉出粉率42.7%,皂苷含量21.4%。该速溶芦笋粉的低、中、高剂量组对小鼠的睡眠发生率(分别为26.7%、36.7%和43.3%)、睡眠潜伏期(分别为29.0、26.3和25.1 min)和睡眠时间(分别为36.4、39.5和46.6 min)均明显优于空白对照组,且呈现出剂量依赖性增加或减少的趋势。研究结果表明,该方法制备的速溶芦笋粉具有一定的改善小鼠睡眠功能的作用。In order to study the enzymatic hydrolysis process conditions of instant asparagus powder and its improvement of sleep function in mice,the market Enyang asparagus was as raw materials,the powder yield and saponin content of instant asparagus powder were as two indicators,and pectinase and cellulas were compounded to enzymatic hydrolysis asparagus after scalded and pulped.The addition of enzyme compound(the mass ratio of pectinase to cellulase was 1:1),enzymolysis temperature,time,and pH were as experimental factors,and the optimum enzymatic hydrolysis process conditions was determined through single factor test and response surface test.Meanwhile,sleep-producing test with subthreshold dose of sodium pentobarbital,sleep latency test and prolonged sleep time test were conducted to investigate its function of improving sleep in mice by comparing the number of sleeping animals and the incidence of sleep,sleep latency and time in each group.The results showed that the optimal enzymolysis conditions for the instant asparagus powder were 0.12%dose of complex enzyme,enzymolysis temperature was 68℃,time was 2.2 h,and pH was 5.2.Under these conditions,the powder yield of instant asparagus powder was 42.7%and the saponin content was 21.4%.The incidence of sleep(26.7%,36.7%and 43.3%respectively),sleep latency(29.0,26.3 and 25.1 min respectively)and sleep time(36.4,39.5 and 46.6 min respectively)in the low,medium and high dose groups of the instant asparagus powder were significantly better than those in the blank control group,and the dose-dependent increase or decrease was observed.The results indicated that the instant asparagus powder prepared by this method could improve the sleep function of mice to some extent.

关 键 词：芦笋粉 酶解工艺 响应面法 改善睡眠 小鼠 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]