原核漆酶的研究进展及其应用  

Research Progress and Application of Prokaryotic Laccase

作　　者：张馨 刘功良[1,2,3] 白卫东 梁景龙[1,2,3] 刘锐 ZHANG Xin;LIU Gongliang;BAI Weidong;LIANG Jinglong;LIU Rui(College of Light Industry and Food Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology,Guangzhou 510225,China;Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food,Ministry of Agriculture,Guangzhou 510225,China)

机构地区：[1]仲恺农业工程学院轻工食品学院,广东广州510225 [2]广东省岭南特色食品科学与技术重点实验室,广东广州510225 [3]农业农村部岭南特色食品绿色加工与智能制造重点实验室,广东广州510225

出　　处：《食品工业科技》2023年第15期451-462,共12页Science and Technology of Food Industry

基　　金：广东省重点建设学科科研能力提升项目(2021ZDJS005);广东省省级农业科技创新及推广项目(No.2023KJ101);广州市科技计划项目(202102020558);广东省企业科技特派员专项资助项目(GDKTP2021036400)。

摘　　要：漆酶可以利用分子氧作为辅助底物,氧化多种酚类或非酚类底物,反应过程中的唯一副产物为水,因而漆酶被认为是一种环保“绿色工具酶”。漆酶在污水处理、造纸、纺织、食品、有机合成、生物制药、检测及生态修复等多个行业具有重要的应用价值。原核漆酶与真菌漆酶相比,往往具有如下的优点:更高的热稳定性和更高的最适反应温度;更适应碱性条件下的催化反应;对抑制剂和金属离子具有更低的敏感性和依赖性;更易于进行异源表达,便于蛋白质工程改造等。因此,近年来,原核漆酶越来越受到人们的关注。本文对原核漆酶的来源、结构、催化过程以及理化性质进行了详细的阐述。并介绍了近些年来原核漆酶的应用及其最新研究进展。A variety of phenolic and non-phenolic substrates can be oxidized by laccase using molecular oxygen as an auxiliary substrate.Due to the fact that laccase produces only water as a by-product,laccase is considered a"green tool enzyme"because it is environmentally friendly.Among the many industries in which laccase is used are sewage treatment,paper making,textiles,food,organic synthesis,biopharmaceuticals,detection,and ecological restoration.Compared to fungal laccase,prokaryotic laccase tends to have the following advantages:higher thermal stability and higher optimum reaction temperature,better suited to catalyse reactions under alkaline conditions,less sensitive and less dependent on inhibitors and metal ions,and more amenable to heterologous expression and protein engineering transformation.As a result,prokaryotic laccase has gained increasing attention in recent years.It describes in detail the origins,structure,catalytic process and physicochemical properties of prokaryotic laccase,its applications,and the recent research progress in this area over the past few years.

关 键 词：原核漆酶 结构 性质 应用 

分 类 号：TS201.2[轻工技术与工程—食品科学]