铁棍山药多糖对产后腰痛运动康复的抗氧化与抗疲劳影响研究  

Effects of polysaccharides from Dioscorea opposita Thunb.cv.Tiegun on anti-oxidation and anti-fatigue during postpartum rehabilitation exercise for lumbago

作　　者：冯斌[1] 杨琦 曹佳[3] FENG Bin;YANG Qi;CAO Jia(Tianjin College,University of Science and Technology Beijing,Tianjin 301830,China;Affiliated Hospital of Hebei University,Baoding 071000,China;Tianjin Vocational College of Bioengineering,Tianjin 300462,China)

机构地区：[1]北京科技大学天津学院,天津301830 [2]河北大学附属医院,河北保定071000 [3]天津生物工程职业技术学院,天津300462

出　　处：《食品与发酵工业》2023年第15期31-37,共7页Food and Fermentation Industries

基　　金：天津市教委科研计划项目(2021KJ067);国家级大学生创新训练项目(202213898012)。

摘　　要：以铁棍山药作为产后腰痛运动康复中重要的糖类营养补充物,通过不同提取方法提取铁棍山药多糖进行体外清除DPPH自由基、·OH能力对比;受试人员定期服用铁棍山药提取溶液,运动后检测受试人员血清丙二醛含量和超氧化物歧化酶活性;监控受试人员运动后次日晨血清睾酮及血清肌酸激酶血液指标,将3组实验结果对比,以进行铁棍山药抗氧化能力与抗疲劳能力科学化评价。结果表明,微波辅助纤维素酶方法在铁棍山药多糖提取当中水平最高为13.83%,微波辅助纤维素酶以及纤维素酶2种方法对于DPPH自由基、·OH具有较强清除能力,但差异不明显。在运动后微波辅助纤维素酶干预组中,血清丙二醛含量降低明显[(3.16±0.59)nmol/mL],超氧化物歧化酶含量升高[(97.35±10.63)nmol/mL]。铁棍山药作为营养补充剂的产后腰痛实验各组,相较于对照组表现出血清睾酮(65.88%)与血清肌酸激酶(55.78%)更快的恢复速度。研究结果可实现铁棍山药多糖的研发与利用,为产后女性运动康复提供营养补充摄入的科学依据。Dioscorea opposita Thunb.cv.Tiegun was used as a carbohydrate nutritional supplement.Different methods were applied to extract polysaccharides,and their scavenging capacities against DPPH radicals and·OH were compared.Subjects who regularly took extract solutions of D.opposita were tested to measure the levels of serum malondialdehyde(MDA)and superoxide dismutase(SOD)after exercise.Post-exercise blood indices such as MDA and serum creatine kinase were monitored the following morning.The results of three types of experiments were compared to evaluate the anti-oxidation and anti-fatigue capacities of D.opposita.The microwave-assisted cellulase method resulted in the highest extraction level of polysaccharides from D.opposita(13.83%),while the microwave-assisted cellulase and the cellulase methods both had strong scavenging ability against DPPH radicals and·OH without significant difference.In the post-exercise microwave-assisted cellulase intervention group,the MDA level decreased significantly[(3.16±0.59)nmol/mL],while that of SOD increased[(97.35±10.63)nmol/mL].In each experimental group where D.opposita was administered as a nutritional supplement to relieve postpartum lumbago,the subjects exhibited a faster recovery rate for serum testosterone(65.58%)and serum creatine kinase(55.78%).This study could provide a scientific basis for the development and utilization of D.opposita as a nutritional supplement for postpartum rehabilitation exercises for women.

关 键 词：铁棍山药 运动康复 抗氧化能力 运动性疲劳 氧自由基代谢 

分 类 号：TS201.4[轻工技术与工程—食品科学]