Ovalbumin-loaded paramagnetic nano-triangles for enhanced dendritic cell stimulation,T_(1)-MR imaging,and antitumor immunity  

作　　者：Yike Hou Zhe Tang Jabeen Farheen Madiha Saeed Lijia Luo Wenzi Ren Dandan Luo Asim Mushtaq Ruibo Zhao Jian Ge Zhangsen Yu Yao Li M.Zubair Iqbal Xiangdong Kong 

机构地区：[1]Institute of Smart Biomedical Materials,School of Materials Science and Engineering,Zhejiang Sci-Tech University,Hangzhou 310018,China [2]State Key Laboratory of Drug Research&Center of Pharmaceutics,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China [3]Cixi Institute of Biomedical Engineering,CAS Key Laboratory of Magnetic Materials and Devices,Key Laboratory of Additive Manufacturing Materials of Zhejiang Province,Ningbo Institute of Materials Technology and Engineering,Chinese Academy of Sciences,Ningbo 315201,China [4]Department of Materials,Imperial College London,London SW72AZ,UK [5]College of Life Sciences,China Jiliang University,Hangzhou 310018,China [6]Laboratory of Nanomedicine,Medical Science Research Center,School of Medicine,Shaoxing University,Shaoxing 312000,China

出　　处：《Journal of Materials Science & Technology》2023年第17期123-137,共15页材料科学技术（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(No.51672250 and 51902289);the Zhejiang International Science and Technology Cooperation Project(Nos.2021C01180 and 2019C04020);the Research Foundation of ZSTU(No.18012134-Y).

摘　　要：Vaccine-based cancer immunotherapy has demonstrated a significant potential for cancer treatment in clinics.Although the efficiencies of vaccines are limited,they can be enhanced by a well-designed antigen delivery system that promotes sufficient antigen presentation of dendritic cells(DCs)for initiating high T cell immunity.Herein,antigen-loaded manganese oxide(Mn_(3)O_(4))triangular-shaped ultrasmall nanoparti-cles were prepared to stimulate DC-based immunotherapy under the guidance of T_(1)magnetic resonance imaging.The FDA-approved triblock copolymer Pluronic^(■)F-68 wasused not onlyto transferthe phase from hydrophobic to hydrophilic but also to enrich antigen loading and improve the biocompatibility of the prepared nanoparticles.Ovalbumin(OVA),a model antigen,was adsorbed on the surface of polymer-coated nanoparticles through electrostatic interaction to form Mn_(3)O_(4)@PF68-OVA nanoparticle-antigen complexes to stimulate DC-based immunization and antigen-specific T cell immunity.The Mn_(3)O_(4)@PF68-OVA nanovaccine(NV)induces negligible toxicity effects against 4T1 and bone marrow-derived dendritic cells(BMDCs)by conventional methods supports the proliferation of intestine organoids,which are an innovative three-dimensional cytotoxicity evaluation system,thereby indicating their potential safety for in vivo cancer therapies.The designed paramagnetic nanovaccine possessed excellent OVA delivery to dendritic-regulated antigen-specific T cells in vitro by stimulating the maturation level of BMDCs.In ad-dition,Mn_(3)O_(4)@PF68-OVA NVs enhance immunity in vivo by increasing the T-cells and M1 macrophages,which suggests improved immunity.Excitingly,vaccination with Mn_(3)O_(4)@PF68-OVA offer complete pro-tection in the prophylactic group and significant tumor inhibition in the therapeutic group against B16-OVA tumor.In addition,the designed nanovaccine demonstrated high T_(1)-MR imaging in the tumor,fur-ther justifying enhanced tumor accumulation and capability to real-time monitor the treatment proce-dure.Th

关 键 词：OVALBUMIN NANOVACCINE Antigen delivery Magnetic resonance imaging Contrast agent Cancer immunotherapy 

分 类 号：R730.5[医药卫生—肿瘤] O657.3[医药卫生—临床医学]