香叶醇及其糖苷在茶树中的积累和对红茶香气的贡献  

The Accumulation of Geraniol and Its Glycoside in Tea Plant and the Contributionto the Aroma of Black Tea

作　　者：周汉琛 杨霁虹 刘亚芹 王辉 雷攀登 Zhou Hanchen;Yang Jihong;Liu Yaqin;Wang Hui;Lei Pandeng(Tea Research Institute,Anhui Academy of Agricultural Sciences,Huangshan 245000,Anhui)

机构地区：[1]安徽省农业科学院茶叶研究所,安徽黄山245000

出　　处：《中国食品学报》2023年第6期339-346,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金青年科学基金项目(32002096);安徽省科技重大专项(20203a06020019);安徽省农业科学创新团队项目(2021YL036);国家现代农业产业技术体系建设专项(CARS-19)。

摘　　要：采用液相质谱联用仪LC-MS、气相质谱联用仪GC-MS分析不同茶树品种、不同组织器官、不同时间香叶醇及其糖苷含量的变化,结果显示:香叶基樱草糖苷在中国变种(Camellia sinensis var. sinensis)中的含量显著高于阿萨姆变种(C.sinensis var. assamica)。香叶基樱草糖苷在茶树幼嫩叶片中含量最高,在根中的含量最低,总体表现出嫩叶>茎>花>根。3月份茶树嫩梢中的香叶醇积累量最高,随着月份的增加而逐渐降低。在祁门红茶加工过程中,香叶基樱草糖苷含量在揉捻阶段显著降低。黄山地区6个品种红茶中,皖茶4号红茶中的香叶醇含量最高;6个品种红茶茶汤中香叶醇的气味活性值(OAV)均>1,分布范围3.93~28.38。Pearson相关性分析显示,有7个萜类合成酶候选基因表达量与香叶基樱草糖苷含量变化呈显著相关。本研究结果为进一步探究香叶醇积累差异成因及形成机制提供理论参考。Geraniol is a monoterpene with a rose trait.This study aims to know the accumulation patterns of geraniol and its glycoside,and the other is to know the contribution of geraniol to the aroma of black tea.The result showed that the CSS(C.sinensis var.sinensis)cultivars possess a higher content of geranyl primeveroside than that in the CSA(C.sinensis var.assamica)cultivars.The amount of geranyl primeveroside in tea young leaf was highest among all tissues of the tea plant,while tea roots had the lowest accumulation of geranyl primeveroside.The fresh leaves of the tea plant in March had a higher accumulation of geraniol compared to those in other months.Furthermore,the results showed that the odor activity value of geraniol in six black tea infusions ranged from 3.93 to 28.38,strongly supporting the potential contribution of geraniol to the aroma of black tea samples.The strong coregulation was obtained between the expression of seven terpene synthase candidate genes and the accumulation of geranyl primeveroside.This work gives insight into the accumulation patterns of geraniol and its primeveroside and promote the research about the biosynthesis and regulation mechanism of geraniol in tea plant.

关 键 词：香叶醇 香叶基樱草糖苷 红茶 气味活性值 萜类合成酶 

分 类 号：TS272.52[农业科学—茶叶生产加工] O657.63[轻工技术与工程—农产品加工及贮藏工程]