高产AI-2信号分子乳酸菌的筛选及生物性能研究  

Studies on Screening of Lactic Acid Bacteria with High Production of AI-2and Its Biological Properties

作　　者：吕欣然 吕孟敏 杨冰艳 顾振辰 刘雪晴 励建荣 白凤翎 檀茜倩 李英美 LüXinran;LüMengmin;Yang Bingyan;Gu Zhenchen;Liu Xueqing;Li Jianrong;Bai Fengling;Tan Xiqian;Li Yingmei(College of Food Science and Technology,Bohai University,National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Food Safety Key Lab of Liaoning Province,Jinzhou 121013,Liaoning;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,Liaoning;Dalian Food Jinzhou Co.,Ltd.,Jinzhou 121019,Liaoning)

机构地区：[1]渤海大学食品科学与工程学院生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心、辽宁省食品安全重点实验室,辽宁锦州121013 [2]大连工业大学海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034 [3]达莲食品(锦州)有限公司,辽宁锦州121019

出　　处：《中国食品学报》2023年第7期152-160,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：辽宁省博士科研启动基金计划项目(2020-BS-236);辽宁省食品安全重点实验室开放课题(LNKLFS202102)。

摘　　要：细菌AI-2信号分子具有调节乳酸菌生物膜形成及益生性等多种功能。本文从16株来源于传统发酵食品和鲤鱼肠道的乳酸菌中,筛选高产AI-2信号分子菌株,并对其生物膜形成,抗氧化活性和产香能力进行分析。结果表明:获得1株高产AI-2菌株DBM2-4,其相对荧光强度达9.69,经生理生化和16S r RNA鉴定为植物乳杆菌。与低产AI-2菌株YF-7相比,菌株DBM2-4经37℃培养24,48,72 h后,生物膜形成量分别高出85.48%,135.54%,63.03%,其完整细胞和无细胞提取物DPPH自由基清除能力分别提高了24.49%和11.37%,风味物质丁二酮和乙醛产量分别增加了3.42 mg/L和5.86 mg/L,说明AI-2信号分子调控植物乳杆菌DBM2-4生物膜形成、抗氧化活性和产香能力。产AI-2信号分子乳酸菌的筛选为改善乳酸菌发酵剂的益生性、抗氧化性和产香能力提供一定的理论基础。AI-2 signal molecule could regulate the biofilm formation and probiotic property of lactic acid bacteria(LAB).In this study,the strain with high production AI-2 were screened from 16 strain lactic acid bacteria derived from traditional fermented food and carp intestines.The ability of biofilm formation,antioxidant and aroma-producing of high production AI-2 LAB were analyzed.The results showed that the relative fluorescence intensity of the high production AI-2 strain DBM2-4 was 9.69.The strain DBM2-4 was identified as Lactiplantibacillus plantarum by physiological and biochemical and 16S rRNA sequencing analysis.Compared with the low production AI-2 strain YF-7,strain DBM2-4 was cultured at 37℃for 24,48 h,and 72 h,the production of biofilm formation increased by 85.48%,135.54%,63.03%,respectively.Meanwhile,the free radical scavenging capacity of DPPH for complete cell and cell-free extracts of strain CY1-2 increased by 24.49%and 11.37%.The production of diacetyl and acetaldehyde increased by 3.42 mg/L and 5.86 mg/L,respectively.These results indicated that AI-2 signal molecule regulated the biofilm formation,antioxidant activity and aroma-producing activity of Lactiplantibacillus plantarum DBM2-4,The screening of LAB with high production of AI-2 would provide a theoretical basis for improving the probiotic,antioxidant and aroma-producing activity of LAB starters.

关 键 词：乳酸菌 AI-2 生物膜 抗氧化 产香 筛选 

分 类 号：TS201.3[轻工技术与工程—食品科学]