罗非鱼鱼皮酶解物及其硒螯合物的结构与抗氧化特性  

Structure and antioxidant properties of tilapia skin hydrolysate and its selenium chelate

作　　者：冯文静 相欢 胡晓[2] 李来好[2] 陈胜军[2] 邓尚贵 FENG Wenjing;XIANG Huan;HU Xiao;LI Laihao;CHEN Shengjun;DENG Shanggui(College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316022,China;Key Lab of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China)

机构地区：[1]浙江海洋大学食品与药学学院,浙江舟山316022 [2]中国水产科学研究院南海水产研究所,农业农村部水产品加工重点实验室,广东广州510300

出　　处：《水产学报》2023年第8期173-186,共14页Journal of Fisheries of China

基　　金：国家特色淡水鱼产业技术体系专项(CARS-46);茂名市引进创新创业团队项目(200201095834980);广东省重点领域研发计划项目(2021B0202060001);广东省企业科技特派员专项(GDKTP2021035300);中国水产科学研究院中央级公益性科研院所基本科研业务费专项(2020TD69);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项(2021SD06);浙江省高层次人才特殊支持计划(2021R51006)。

摘　　要：为了高值化利用罗非鱼副产物资源以及为酶解物-硒螯合物的产业化提供理论依据,本实验以罗非鱼鱼皮胶原蛋白(TSC)为原料,用4种蛋白酶对其进行酶解,分别获得了酸性蛋白酶酶解物(TSCAc)、木瓜蛋白酶酶解物(TSCP)、菠萝蛋白酶酶解物(TSCB)、碱性蛋白酶酶解物(TSCAl),以Na2SeO3为硒源,制备并筛选出硒螯合量最高的酶解物,测定酶解物及其硒螯合物的紫外光谱、荧光光谱、红外光谱、表面疏水性,并对酶解物及其硒螯合物的抗氧化性与抗氧化稳定性进行分析。结果显示,TSCAl的水解度(DH)最高(18.2%),其与硒螯合后的硒螯合量最高(153.5 mg/g),硒螯合率最高(23.7%),小分子量(500~1000 u)的含量最高。其与硒螯合后,紫外光谱吸收峰强度增大,荧光光谱吸收峰由波长419.8 nm红移到422.0 nm且荧光强度减弱,疏水性在螯合后降低,表明TSCAl与硒螯合生成了新的物质。推测酰胺键、氨基的氮原子、羧基的氧原子可能参与了与硒的螯合反应。研究表明,碱性蛋白酶酶解物-硒螯合物(TSCAl-Se)具有良好的抗氧化性,其在不同温度、pH值以及经胃消化后皆具有稳定的抗氧化活性。本研究制备了一种新型罗非鱼鱼皮胶原蛋白酶解物-硒螯合物,为研究和开发硒元素膳食补充剂及抗氧化剂提供了理论依据。Selenium is one of the essential trace elements in the human body.Lack of selenium can lead to many diseases,such as Keshan disease,large bone disease and so on.Compared with inorganic selenium,organic selenium can cross the intestinal wall actively,so it is necessary to develop organic selenium with high biological activity.Selenium chelate,a collagen protease hydrolysate,has good antioxidant properties,can also supplement selenium and collagen for human body,and has high bioavailability.In order to make efficient use of tilapia by-product resources and provide theoretical basis for the industrialization of selenium chelate,tilapia skin collagen(TSC)was used as raw material for enzymatic hydrolysis by 4 kinds of proteases.The enzymolysis products of acid protease(TSCAc),papain enzymolysis product(TSCP),bromelain enzymolysis product(TSCB)and alkaline protease enzymolysis product(TSCAl)were obtained.Using Na_(2)SeO_(3) as the source of selenium,the hydrolysate with the highest selenium binding amount was prepared and screened.UV,fluorescence,FTIR and surface hydrophobicity of the enzyme hydrolysate and its selenium chelate were determined,and the antioxidant activity and antioxidant stability of the enzyme hydrolysate and its selenium chelate were analyzed.The hydrolysate of alkaline protease(TSCAl)had the highest hydrolysis degree(18.2%),and the proportion of molecular weight<1000 u was 53.7%,which was higher than that of other hydrolysates.The chelate of alkaline protease(TSCAl-Se)had the highest selenium binding amount(153.5 mg/g)and the selenium binding rate was 23.7%.After the chelation of TSCAl and selenium,the intensity of the absorption band at 210-238 nm wavelength decreased and showed a blue shift,and the intensity of the absorption band at 238 nm-300 nm wavelength increased.After the chelation of TSCAl and selenium,the fluorescence intensity of the fluorescence spectrum weakened,and the absorption peak red shifted 2 nm.After TSCAl chelated with selenium,in the infrared spectrum,the absorption peak of N-

关 键 词：罗非鱼 鱼皮 胶原蛋白 硒 酶解物 螯合物 结构 抗氧化 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程] S985[轻工技术与工程—食品科学与工程]