茶碱和可可碱对高脂饮食小鼠体质量和胰岛素抵抗的影响  

Effects of Theophylline and Theobromine on Body Mass and Insulin Resistance in Mice Fed with High-Fat Diet

作　　者：董金娅 何小芳 杜晓翠[1] 方崇业 DONG Jinya;HE Xiaofang;DU Xiaocui;FANG Chongye(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,Yunnan,China;Yunnan Research Center for Advanced Tea Processing,Kunming 650201,Yunnan,China;College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,Yunnan,China)

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南省茶深加工工程技术研究中心,云南昆明650201 [3]云南农业大学食品科学技术学院,云南昆明650201

出　　处：《食品研究与开发》2023年第16期68-75,共8页Food Research and Development

基　　金：云南省科技厅科技计划项目(202203AP140011)。

摘　　要：为研究茶碱和可可碱对高脂饮食诱导小鼠体质量和胰岛素抵抗的影响,选取50只健康的C57BL/6J小鼠,随机分为低脂饮食(low-fat diet,LFD)组、LFD+茶碱组、LFD+可可碱组、高脂饮食(high-fat diet,HFD)组、HFD+茶碱组、HFD+可可碱组。每周定期检测小鼠体质量、空腹血糖水平、摄食量和饮水量,14周和15周进行腹腔葡萄糖耐量试验(intraperitoneal glucose tolerance test,IPGTT)和腹腔胰岛素耐受试验(intraperitoneal insulin tolerance test,IPITT),饲养结束后测定脏器系数和血清生化指标。结果显示:茶碱能有效降低小鼠体质量(低于LFD组4.89%~16.28%,HFD组7.86%~30.81%)和减少脂肪堆积;IPGTT和IPITT表明茶碱能改善小鼠葡萄糖耐受性和缓解胰岛素抵抗;与HFD组相比,茶碱能降低小鼠血清总胆固醇、甘油三酯、低密度脂蛋白和谷丙转氨酶水平,但可可碱没有发挥这些作用。综上,茶碱和可可碱虽互为同分异构体的甲基黄嘌呤生物碱,但对HFD诱导小鼠在减肥降脂方面的影响相差较大。其中,茶碱能抑制HFD诱导小鼠的体质量增加和缓解胰岛素抵抗,而可可碱没有产生此类效果。The effects of theophylline and theobromine on the body weight and insulin resistance of the mice fed with a high-fat diet(HFD)were studied.Fifty healthy C57BL/6J mice were selected and randomly divided into low-fat diet(LFD)group,LFD+theophylline group,LFD+theobromine group,high-fat diet(HFD)group,HFD+theophylline group,and HFD+theobromine group.The body mass,fasting blood glucose level,food intake,and water intake were recorded every week.The intraperitoneal glucose tolerance test(IPGTT)and intraperitoneal insulin tolerance test(IPITT)were performed at weeks 14 and 15.Organ indexes and serum biochemical indexes were determined at the end of the feeding treatment.The results showed that theophylline reduced the body mass by 4.89%-16.28%compared with the LFD group and by 7.86%-30.81%compared with the HFD group and inhibited fat accumulation.The results of IPGTT and IPITT showed that theophylline improved glucose tolerance and alleviated insulin resistance in mice.Compared with the HFD group,theophylline lowered the serum levels of total cholesterol,triglycerides,low-density lipoprotein,and alanine aminotransferase in mice,while theobromine did not exert these effects.In conclusion,although theophylline and theobromine,as methylxanthine alkaloids,are isomers of each other,their effects on weight loss and lipid reduction in HFD-induced mice are different.Theophylline can reduce the body mass gain and alleviate insulin resistance in HFD-induced mice,while theobromine did not show such effects.

关 键 词：茶碱 可可碱 高脂饮食 体质量 胰岛素抵抗 

分 类 号：TS201.4[轻工技术与工程—食品科学]