紫苏籽粕迷迭香酸的亚临界水提取工艺优化及其抗氧化活性  

Optimization of Subcritical Water Extraction of Rosmarinic Acid from Perilla(Perilla frutescens L.)Seed Meal and Its Antioxidant Activity

作　　者：闫林林[1] 王艳辉 张佳婵[2] 郑光耀[1] YAN Linlin;WANG Yanhui;ZHANG Jiachan;ZHENG Guangyao(Institute of Chemical Industry of Forest Products,CAF,National Engineering Lab.for Biomass Chemical Utilization,Key Lab.of Chemical Engineering of Forest Products,National Forestry and Grassland Administration,Key Lab.of Biomass Energy and Material,Jiangsu Province,Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing 210042,China;Beijing Key Lab.of Plant Resource Research and Development,Beijing Technology and Business University,Beijing 100048,China)

机构地区：[1]中国林业科学研究院林产化学工业研究所,生物质化学利用国家工程实验室,国家林业和草原局林产化学工程重点实验室,江苏省生物质能源与材料重点实验室,江苏省林业资源高效加工利用协同创新中心,江苏南京210042 [2]北京工商大学,北京市植物资源研究开发重点实验室,北京100048

出　　处：《食品工业科技》2023年第17期176-185,共10页Science and Technology of Food Industry

基　　金：国家自然科学基金(31901236);北京工商大学北京市植物资源研究开发重点实验室开放课题基金(PRRD-2018-YB8)。

摘　　要：本研究采用绿色环保的亚临界水提取技术从紫苏籽粕中提取迷迭香酸。在单因素实验基础上,采用3因素3水平的响应面分析法,以迷迭香酸的提取得率为响应值,以提取温度(140~180℃)、提取时间(20~40 min)、料液比(1:30~1:50 g/mL)为自变量,确定亚临界水提取的最佳工艺参数。同时,比较了亚临界水提取法和几种常规提取法对迷迭香酸提取得率及其抗氧化活性(DPPH和ABTS自由基清除力)的影响。结果表明,优化后的亚临界水提取工艺参数为:提取温度163℃、提取时间30 min、料液比1:41 g/mL;此条件下,迷迭香酸的提取得率为4.91±0.16 mg/g dw,其清除DPPH和ABTS自由基能力的V_(C)当量分别为10.62和13.46 mgV_(C)/g dw,均显著高(P<0.05)于几种常规提取法。此外,扫描电子显微镜(SEM)结果显示,亚临界水提取后的紫苏籽粕粉末结构更疏松,说明亚临界水更有利于迷迭香酸的提取。Subcritical water extraction(SWE),a green and environmentally friendly extraction technology,was successfully used to extract rosmarinic acid from Perilla seed meal(PSM).Based on the results of single-factor experiments,three-factor and three-level of response surface methodology were used to optimize the extraction parameters of rosmarinic acid in PSM,including extraction temperature(140~180℃),extraction time(20~40 min)and solid-liquid ratio(1:30~1:50 g/mL).Meanwhile,the effects of SWE and several conventional extraction methods on the extraction yields of rosmarinic acid and their antioxidant activities(DPPH and ABTS free radical scavenging capacity)of PSM extracts were compared.The results showed that the optimized parameters were as follows:Extraction temperature 163℃,extraction time 30 min,and the ratio of solid to liquid 1:41 g/mL.Under these conditions,the extraction yield of rosmarinic acid was 4.91±0.16 mg/g dw,and the free radicals scavenging capacities of DPPH and ABTS were 10.62 and 13.46 mgV_(C)/g dw,respectively,which were significantly higher than those of several conventional extraction methods.In addition,scanning electron microscope(SEM)showed that the structure of the PSM residues became looser after SWE,which could benefit the extraction of rosmarinic acid.

关 键 词：亚临界水 紫苏籽粕 迷迭香酸 响应面 抗氧化活性 

分 类 号：TS209[轻工技术与工程—食品科学]