2017—2021年河南省肉与肉制品食源性致病菌监测及部分肠杆菌耐药特征分析  

Prevalence of foodborne pathogens and antimicrobial resistance characteristics of Enterobacteriaceae in meat products in He’nan Province from 2017 to 2021

作　　者：吴玲玲[1] 李艳芬[1] 炊慧霞[1] 崔莹[1] 张秀丽[1] 李辉[1] 李永利[1] 廖兴广[1] WU Lingling;LI Yanfen;CHUI Huixia;CUI Ying;ZHANG Xiuli;LI Hui;LI Yongli;LIAO Xingguang(He’nan Provincial Center for Disease Control and Prevention,He’nan Zhengzhou 450016,China)

机构地区：[1]河南省疾病预防控制中心,河南郑州450016

出　　处：《中国食品卫生杂志》2023年第5期749-756,共8页Chinese Journal of Food Hygiene

基　　金：河南省医学科技攻关省部共建青年项目(SBGJ202103030);河南省医学科技攻关项目(LHGJ20190690)。

摘　　要：目的了解河南省肉制品中食源性致病菌的污染状况,并对肠杆菌进行血清学分型和耐药特征分析。方法收集2017—2021年河南省郑州市等21个监测点的1934份肉与肉制品,参考《国家食品污染物和有害因素风险监测工作手册》和《河南省食品微生物风险监测工作手册》进行沙门菌、致泻大肠埃希菌、小肠结肠炎耶尔森菌、产气荚膜梭菌、单核细胞增生李斯特菌、金黄色葡萄球菌和空肠弯曲菌等7种食源性致病菌的检测,使用微量肉汤稀释法对致泻大肠埃希菌和沙门菌进行药敏试验,分析耐药率和耐药谱等特征。结果1934份样品中检出478株致病菌,其中沙门菌96株,共26个血清型,以肠炎沙门菌为主;致泻大肠埃希菌82株,以肠集聚性大肠埃希菌为主;产气荚膜梭菌68株,小肠结肠炎耶尔森菌38株,空肠弯曲菌33株,单核细胞增生李斯特菌140株和金黄色葡萄球菌21株。致病菌总检出率为24.72%,调理肉制品检出率最高达到89.00%,其次为生禽肉(36.79%)和生畜肉(22.35%)。96株沙门菌对氨苄西林(71.88%)、萘啶酸(63.54%)及四环素(58.33%)高水平耐药;82株致泻大肠埃希菌对四环素(84.38%)、氨苄西林(67.19%)及氯霉素(64.06%)高水平耐药,且普遍存在多重耐药情况。结论河南省肉与肉制品存在不同程度的食源性致病菌污染,尤其是调理肉制品污染较重。致泻大肠埃希菌和沙门菌普遍对氨苄西林耐药,且存在多重耐药情况。为减少食源性疾病的暴发,降低感染率,应加强对肉与肉制品的监管监控,以保障公众食品安全。Objective To investigate the contamination status of foodborne pathogens in meat products in He’nan Province and analyze the serotyping and drug resistance characteristics of Enterobacteriaceae.Methods A total of 1934 samples of meat products were collected from 21 surveillance sites,including Zhengzhou City,He’nan Province,from 2017 to 2021.Seven foodborne pathogens,including Salmonella,Diarrheogenic Escherichia coli(DEC),Yersinia enterocolitica,Clostridium perfringens,Listeria monocytogenes,Staphylococcus aureus,and Campylobacter jejuni were detected according to the National Food Contamination and Hazardous Factors Risk Monitoring Manual and the He’nan Food Safety Risk Monitoring Program.The drug susceptibility of DEC and Salmonella was tested using the broth microdilution method to analyze the drug resistance rate,spectrum,and other features.Results Four hundred and seventy eight strains of pathogenic bacteria were detected in 1934 samples,including 96 strains of Salmonella of 26 serotypes,mainly Salmonella enteritidis;82 strains of DEC,with a predominant pathotype of Enteroaggregative Escherichia coli(EAEC);68 strains of Clostridium perfringens;38 strains of Yersinia enterocolitica;33 strains of Campylobacter jejuni;140 strains of Listeria monocytogenes;and 21 strains of Staphylococcus aureus.The total detection rate of pathogenic bacteria was 24.72%;for prepared meat products,it was 89.00%,followed by raw poultry meat at 36.79%and raw livestock meat at 22.35%.The highest antibiotic resistance rates of the 96 strains of Salmonella were for ampicillin(71.88%),nalidixic acid(63.54%),and tetracycline(58.33%).Eighty-two DEC strains were resistant to tetracycline(84.38%),ampicillin(67.19%),and chloramphenicol(64.06%),with widespread multiple drug resistance.Conclusion The meat products of He’nan Province were contaminated by pathogenic bacteria to varying degrees,especially in prepared meat products.The most common Enterobacteriaceae were DEC and Salmonella,which were mostly resistant to ampicillin and sh

关 键 词：食源性致病菌 肉与肉制品 肠杆菌 血清分型 耐药特征分析 

分 类 号：R155[医药卫生—营养与食品卫生学]