灵芝固态发酵三七渣-城市污泥产漆酶的培养基优化  

Optimization of medium for laccase production from solid-state fermentation of Panax notoginseng residues and municipal sludge by Ganoderma lucidum

作　　者：谭显东[1] 陶红群 张韬 吉栗漫 魏琨 陈星颖 陈香楚 TAN Xiandong;TAO Hongqun;ZHANG Tao;JI Liman;WEI Kun;CHEN Xingying;CHEN Xiangchu(College of Resources and Environment,Chengdu University of Information Technology,Chengdu 610225,China;Chengdu Research Institute of Environmental Protection Science,Chengdu 610072,China)

机构地区：[1]成都信息工程大学资源环境学院,四川成都610225 [2]成都市环境保护科学研究院,四川成都610072

出　　处：《中国酿造》2023年第8期220-225,共6页China Brewing

基　　金：四川省重点研发计划项目(2021YFG0263);成都市重点研发支撑计划(2019-YF05-02457-SN)。

摘　　要：为了实现固体废弃物的资源化利用,提高灵芝固态发酵三七渣-城市污泥混合物产漆酶的酶活,该研究在单因素试验的基础上,以磷酸二氢钾添加量、硫酸铜添加量、城市污泥占比为自变量,漆酶酶活为响应值,采用响应面分析法优化了三七渣-城市污泥固态发酵产漆酶的培养基组成。结果表明,最佳培养基组成为磷酸二氢钾添加量0.3%、硫酸铜添加量0.05%、城市污泥占比30%。在此条件下进行验证试验,得到的漆酶酶活为5.90 IU/g,比优化前提高了1.01 IU/g。In order to realize the resource utilization of solid waste and improve the enzyme activity of laccase produced by solid-state fermentation of Panax notoginseng residue and municipal sludge mixture by Ganoderma lucidum.On the basis of single factor experiment,using potassium dihydrogen phosphate addition,copper sulfate addition and the proportion of municipal sludge as the independent variable,the laccase activity as the response value,the composition of medium for laccase production by solid fermentation of P.notoginseng residue-municipal sludge was optimized by response surface analysis.The results showed that the optimum media composition was as follows:potassium dihydrogen phosphate 0.3%,copper sulfate 0.05%,and the ratio of municipal sludge 30%.Under these conditions,the laccase activity was 5.90 IU/g,which was 1.01 IU/g higher than before optimization.

关 键 词：三七渣 固态发酵 漆酶 灵芝 响应面分析 

分 类 号：TS201.3[轻工技术与工程—食品科学] R284.2[轻工技术与工程—食品科学与工程]